EC Number | Cloned (Comment) | Organism |
---|---|---|
1.5.1.3 | overexpression of wild-type and mutant enzymes in Escherichia coli | Haloferax volcanii |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.5.1.3 | A31K | site-directed mutagenesis | Haloferax volcanii |
1.5.1.3 | L30K | site-directed mutagenesis | Haloferax volcanii |
1.5.1.3 | L30K/A31K | site-directed mutagenesis | Haloferax volcanii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.3 | additional information | - |
additional information | overview Km-values of wild-type and mutant enzymes decreasing with increasing concentration of KCl | Haloferax volcanii | |
1.5.1.3 | 0.08 | - |
7,8-dihydrofolate | lowest Km value in 3.0 M KCl, recombinant wild-type enzyme | Haloferax volcanii |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.3 | Haloferax volcanii | - |
extremely halophilic archaebacterium | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.1.3 | large quantities of recombinant wild-type enzyme from Escherichia coli, purification of mutants L30K, A31K and double mutant L30K/A31K recombinant from Escherichia coli | Haloferax volcanii |
EC Number | Renatured (Comment) | Organism |
---|---|---|
1.5.1.3 | very fast and total reconstitution of active recombinant wild-type enzyme from inclusion bodies due to overexpression in E. coli by 6 M guanidine hydrochloride followed by dilution into 1 M NaCl or KCl solution | Haloferax volcanii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.3 | 7,8-dihydrofolate + NADPH | - |
Haloferax volcanii | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.3 | 3.14 | - |
7,8-dihydrofolate | recombinant wild-type enzyme | Haloferax volcanii | |
1.5.1.3 | 4.97 | - |
7,8-dihydrofolate | recombinant mutant A31K | Haloferax volcanii | |
1.5.1.3 | 9.5 | - |
7,8-dihydrofolate | recombinant double mutant L30K/A31K | Haloferax volcanii | |
1.5.1.3 | 100 | - |
7,8-dihydrofolate | recombinant mutant L30K | Haloferax volcanii |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.3 | NADPH | - |
Haloferax volcanii |