EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.5.1.3 | additional information | selection of cell line with altered enzyme due to decreased methotrexate-sensitivity | Mus musculus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.3 | methasquin | - |
Mus musculus | |
1.5.1.3 | methotrexate | wild-type | Mus musculus | |
1.5.1.3 | pyrimethamine | - |
Mus musculus | |
1.5.1.3 | trimethoprim | folate antagonist drug | Mus musculus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.3 | 0.0022 | - |
NADPH | altered enzyme | Mus musculus | |
1.5.1.3 | 0.003 | - |
7,8-dihydrofolate | wild-type enzyme | Mus musculus | |
1.5.1.3 | 0.01 | - |
7,8-dihydrofolate | altered enzyme | Mus musculus | |
1.5.1.3 | 0.03 | - |
NADPH | wild-type | Mus musculus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.3 | Mus musculus | - |
altered enzyme encoded by amplified genes in cultured fibroblasts | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.5.1.3 | fibroblast | cultured 3T6 mouse embryo fibroblasts | Mus musculus | - |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.1.3 | additional information | - |
1 mM NADPH protects against inactivation | Mus musculus |
1.5.1.3 | 55 | - |
half-life: 8 min | Mus musculus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.3 | 0.243 | - |
7,8-dihydrofolate | altered enzyme | Mus musculus | |
1.5.1.3 | 4.67 | - |
7,8-dihydrofolate | wild-type enzyme | Mus musculus |