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Literature summary extracted from

  • D'Souza, L.; Warwick, P.E.; Freisheim, J.H.
    Purification and properties of dihydrofolate reductase from an amethopterin-resistant strain of Streptococcus faecium (1972), Biochemistry, 11, 1528-1534.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.3 methopterin
-
 Enterococcus faecium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.3 additional information
-
 additional information
-
 Enterococcus faecium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.3 20000
-
 sedimentation equilibrium analysis and gel filtration Enterococcus faecium

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.3 Enterococcus faecium
-
 amethopterin-resistant strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.3 amethopterin-resistant strain Enterococcus faecium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.3 300
-
 amethopterin-resistant cells, purified enzyme Enterococcus faecium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.3 7,8-dihydrofolate + NADPH
-
 Enterococcus faecium 5,6,7,8-tetrahydrofolate + NADP+
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.1.3 100
-
 7,8-dihydrofolate form I Enterococcus faecium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.3 6.5
-
-
 Enterococcus faecium

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.3 NADPH
-
 Enterococcus faecium