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Literature summary extracted from

  • Smith, D.D.S.; Wood, N.J.; Hodgson, D.A.
    Interaction between primary and secondary metabolism in Streptomyces coelicolor A3(2): role of pyrroline-5-carboxylate dehydrogenase (1995), Microbiology, 141, 1739-1744.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.88 additional information no effect of proline on activity Streptomyces coelicolor

Application

EC Number Application Comment Organism
1.2.1.88 medicine investigation of regulation of antibiotic production, i.e. undecylprodigiosin and related compounds, in secondary metabolism from proline as amino acid precursor Streptomyces coelicolor

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.88 L-Glu very weak inhibitor Streptomyces coelicolor
1.2.1.88 additional information no effect of proline on activity Streptomyces coelicolor
1.2.1.88 NADH product inhibition Streptomyces coelicolor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.88 0.043
-
 NAD+
-
 Streptomyces coelicolor
1.2.1.88 0.109
-
 1-pyrroline-5-carboxylate
-
 Streptomyces coelicolor

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.88 68000
-
 4 * 68000, SDS-PAGE Streptomyces coelicolor
1.2.1.88 265000 305000 native PAGE and gel filtration Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.88 1-pyrroline-5-carboxylate + NAD+ + H2O Streptomyces coelicolor
-
 L-glutamate + NADH
-
 ?
1.2.1.88 1-pyrroline-5-carboxylate + NAD+ + H2O Streptomyces coelicolor A3(2)
-
 L-glutamate + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.88 Streptomyces coelicolor
-
-
-
1.2.1.88 Streptomyces coelicolor A3(2)
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.88
-
 Streptomyces coelicolor

Reaction

EC Number Reaction Comment Organism Reaction ID
1.2.1.88 L-glutamate 5-semialdehyde + NAD+ + H2O = L-glutamate + NADH + H+ induction by proline, activation on gene transcription level Streptomyces coelicolor
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.88 22
-
-
 Streptomyces coelicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.88 1-pyrroline-5-carboxylate + NAD+ + H2O
-
 Streptomyces coelicolor L-glutamate + NADH
-
 ?
1.2.1.88 1-pyrroline-5-carboxylate + NAD+ + H2O
-
 Streptomyces coelicolor A3(2) L-glutamate + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.88 tetramer 4 * 68000, SDS-PAGE Streptomyces coelicolor

Synonyms

EC Number Synonyms Comment Organism
1.2.1.88 P5C dehydrogenase
-
 Streptomyces coelicolor

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.88 25
-
 1-pyrroline-5-carboxylate
-
 Streptomyces coelicolor

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.88 NAD+
-
 Streptomyces coelicolor

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.2.1.88 0.6
-
 NADH product inhibition, with NAD+ Streptomyces coelicolor
1.2.1.88 0.77
-
 NADH product inhibition, with 1-pyrroline-5-carboxylate Streptomyces coelicolor