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Literature summary extracted from

  • Smirnova, I.P.; Berezov, T.T.
    Substrate specificity of L-amino acid oxidases of certain strains of the genus Trichoderma (1989), Mikrobiologiya, 58, 49-53.
No PubMed abstract available

Application

EC Number Application Comment Organism
1.4.3.14 medicine antitumor activity Trichoderma viride
1.4.3.14 medicine antitumor activity Trichoderma harzianum
1.4.3.14 medicine antitumor activity Trichoderma longibrachiatum
1.4.3.14 medicine antitumor activity Trichoderma aureoviride

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.14 Trichoderma aureoviride
-
-
-
1.4.3.14 Trichoderma harzianum
-
-
-
1.4.3.14 Trichoderma longibrachiatum
-
-
-
1.4.3.14 Trichoderma viride
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.14 rapid loss of activity during isolation and purification Trichoderma viride
1.4.3.14 rapid loss of activity during isolation and purification Trichoderma harzianum
1.4.3.14 rapid loss of activity during isolation and purification Trichoderma longibrachiatum
1.4.3.14 rapid loss of activity during isolation and purification Trichoderma aureoviride

Reaction

EC Number Reaction Comment Organism Reaction ID
1.4.3.14 L-lysine + O2 + H2O = 6-amino-2-oxohexanoate + NH3 + H2O2 the substrate specificity of Trichoderma strains changes as a function of the age of the culture and its morphological condition Trichoderma viride
a
1.4.3.14 L-lysine + O2 + H2O = 6-amino-2-oxohexanoate + NH3 + H2O2 the substrate specificity of Trichoderma strains changes as a function of the age of the culture and its morphological condition Trichoderma harzianum
a
1.4.3.14 L-lysine + O2 + H2O = 6-amino-2-oxohexanoate + NH3 + H2O2 the substrate specificity of Trichoderma strains changes as a function of the age of the culture and its morphological condition Trichoderma longibrachiatum
a
1.4.3.14 L-lysine + O2 + H2O = 6-amino-2-oxohexanoate + NH3 + H2O2 the substrate specificity of Trichoderma strains changes as a function of the age of the culture and its morphological condition Trichoderma aureoviride
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.14 additional information
-
-
 Trichoderma viride
1.4.3.14 additional information
-
 the magnitude of the L-lysine-alpha-oxidase activity is demonstrated as a function of the sources of carbon and nitrogen, the number of conidia forming during the growth is correlates completely with the magnitude of the L-lysine-alpha-oxidase activity Trichoderma harzianum

Storage Stability

EC Number Storage Stability Organism
1.4.3.14 -4°C, practically no loss of activity over several months Trichoderma viride
1.4.3.14 -4°C, practically no loss of activity over several months Trichoderma harzianum
1.4.3.14 -4°C, practically no loss of activity over several months Trichoderma longibrachiatum
1.4.3.14 -4°C, practically no loss of activity over several months Trichoderma aureoviride
1.4.3.14 4°C, activity preserved Trichoderma viride
1.4.3.14 4°C, activity preserved Trichoderma harzianum
1.4.3.14 4°C, activity preserved Trichoderma longibrachiatum
1.4.3.14 4°C, activity preserved Trichoderma aureoviride

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.14 additional information very low activity with DL-phenylalanine, L-histidine, L-arginine, DL-methionine and L-glycine Trichoderma aureoviride ?
-
 ?
1.4.3.14 additional information very low activity with DL-phenylalanine and DL-methionine Trichoderma harzianum ?
-
 ?
1.4.3.14 additional information very low activity with several other amino acids Trichoderma viride ?
-
 ?
1.4.3.14 additional information no activity with L-cysteine, L-asparagine-NH2, DL-serine, DL-asparagine and DL-glutamic acid Trichoderma viride ?
-
 ?
1.4.3.14 additional information no activity with L-cysteine, L-asparagine-NH2, DL-serine, DL-asparagine and DL-glutamic acid Trichoderma harzianum ?
-
 ?
1.4.3.14 additional information no activity with L-cysteine, L-asparagine-NH2, DL-serine, DL-asparagine and DL-glutamic acid Trichoderma longibrachiatum ?
-
 ?
1.4.3.14 additional information no activity with L-cysteine, L-asparagine-NH2, DL-serine, DL-asparagine and DL-glutamic acid Trichoderma aureoviride ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.3.14 37
-
 assay at Trichoderma viride
1.4.3.14 37
-
 assay at Trichoderma harzianum
1.4.3.14 37
-
 assay at Trichoderma longibrachiatum
1.4.3.14 37
-
 assay at Trichoderma aureoviride

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.4.3.14 additional information
-
 the Trichoderma strains show different amino acid oxidation activity over a varied pH range Trichoderma viride
1.4.3.14 additional information
-
 the Trichoderma strains show different amino acid oxidation activity over a varied pH range Trichoderma harzianum
1.4.3.14 additional information
-
 the Trichoderma strains show different amino acid oxidation activity over a varied pH range Trichoderma longibrachiatum
1.4.3.14 additional information
-
 the Trichoderma strains show different amino acid oxidation activity over a varied pH range Trichoderma aureoviride