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Literature summary extracted from

  • Carlberg, I.; Norlund, S.
    Purification and partial characterization of glutamate synthase from Rhodospirillum rubrum grown under nitrogen-fixing conditions (1991), Biochem. J., 279, 151-154.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.13 6-diazo-5-oxo-L-norleucine 35% inhibition at 0.0025 mM, when reaction is initiated with 0.5 mM L-glutamine, 73% inhibition at 0.0025 mM, when reaction is initiated with 2.5 mM 2-oxoglutarate Rhodospirillum rubrum
1.4.1.13 azaserine 38% inhibition at 0.0125 mM when reaction is initiated with 0.5 mM L-glutamine and 69% inhibition at 0.0125 mM when reaction is initiated with 2.5 mM 2-oxoglutarate Rhodospirillum rubrum
1.4.1.13 L-aspartate
-
 Rhodospirillum rubrum
1.4.1.13 L-methionine
-
 Rhodospirillum rubrum
1.4.1.13 NADP+ competitive inhibitor with NADPH, 54% inhibition at 1 mM Rhodospirillum rubrum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.13 0.01
-
 2-oxoglutarate
-
 Rhodospirillum rubrum
1.4.1.13 0.016
-
 NADPH
-
 Rhodospirillum rubrum
1.4.1.13 0.065
-
 L-glutamine
-
 Rhodospirillum rubrum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.4.1.13 Iron iron-sulfur protein Rhodospirillum rubrum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.13 53000
-
 x * 152000 + x * 53000, SDS-PAGE Rhodospirillum rubrum
1.4.1.13 152000
-
 x * 152000 + x * 53000, SDS-PAGE Rhodospirillum rubrum
1.4.1.13 800000
-
 gel filtration, presence of Cl- Rhodospirillum rubrum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.13 L-glutamine + 2-oxoglutarate + NADPH Rhodospirillum rubrum main route for assimilation of ammonium compounds L-glutamate + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.13 Rhodospirillum rubrum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.13 using ammonium sulfate precipitation and column chromatography on DEAE-Sepharose, 2',5'-ADP-Sepharose and Sephacryl S-300 Rhodospirillum rubrum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.13 21
-
-
 Rhodospirillum rubrum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.13 L-glutamine + 2-oxoglutarate + NADPH main route for assimilation of ammonium compounds Rhodospirillum rubrum L-glutamate + NADP+
-
 ?
1.4.1.13 L-glutamine + 2-oxoglutarate + NADPH + H+ no activity with NH4+ Rhodospirillum rubrum L-glutamate + NADP+
-
 ?
1.4.1.13 NH3 + 2-oxoglutarate + NADPH + H+ the activity with 10 mM NH4+ ions is less than 2% that with L-glutamine Rhodospirillum rubrum L-glutamate + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.1.13 ? x * 152000 + x * 53000, SDS-PAGE Rhodospirillum rubrum

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.13 flavin flavoenzyme Rhodospirillum rubrum
1.4.1.13 NADH no activity with NADH Rhodospirillum rubrum
1.4.1.13 NADPH
-
 Rhodospirillum rubrum