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Literature summary extracted from

  • Sung, H.C.; Tachiki, T.; Kumagai, H.; Tochikura, T.
    Properties of glutamate synthase from Brevibachterium flavum (1984), J. Ferment. Technol., 62, 569-575.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.4.1.13 alpha-ketoglutarate stabilizes [Brevibacterium] flavum
1.4.1.13 glutamine stabilizes [Brevibacterium] flavum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.13 2'-adenylic acid
-
 [Brevibacterium] flavum
1.4.1.13 AgNO3 at 0.001 mM completely inhibits [Brevibacterium] flavum
1.4.1.13 ATP at 3.5 mM 12% inhibition [Brevibacterium] flavum
1.4.1.13 D-glutamate at 3.5 mM 10% inhibition [Brevibacterium] flavum
1.4.1.13 D-Lysine significantly inhibits [Brevibacterium] flavum
1.4.1.13 HgCl2 at 0.001 mM remains 8% of activity [Brevibacterium] flavum
1.4.1.13 iodoacetate completely inhibits at 1 mM, 2-mercaptoethanol protects against inhibition [Brevibacterium] flavum
1.4.1.13 L-glutamate at 3.5 mM 13% inhibition [Brevibacterium] flavum
1.4.1.13 L-histidine significant inhibitor [Brevibacterium] flavum
1.4.1.13 L-homoserine significant inhibitor [Brevibacterium] flavum
1.4.1.13 L-malate at 3.5 mM 67% inhibition [Brevibacterium] flavum
1.4.1.13 L-methionine
-
 [Brevibacterium] flavum
1.4.1.13 lysine significantly inhibits [Brevibacterium] flavum
1.4.1.13 methionine sulfone
-
 [Brevibacterium] flavum
1.4.1.13 NADH at 3.5 mM 31% inhibition [Brevibacterium] flavum
1.4.1.13 NADP+ 76% inhibition [Brevibacterium] flavum
1.4.1.13 oxalglycine
-
 [Brevibacterium] flavum
1.4.1.13 p-chloromercuribenzoate completely inhibits at 1 mM, 2-mercaptoethanol protects against inhibition [Brevibacterium] flavum
1.4.1.13 phenylalanine significantly inhibits [Brevibacterium] flavum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.13 0.06
-
 NADPH
-
 [Brevibacterium] flavum
1.4.1.13 0.06
-
 alpha-ketoglutarate
-
 [Brevibacterium] flavum
1.4.1.13 0.24
-
 L-glutamine
-
 [Brevibacterium] flavum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.4.1.13 Iron enzyme contains 8 mol of non-heme iron [Brevibacterium] flavum

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.13 [Brevibacterium] flavum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.13 using ammonium sulfate fractionation, column chromatography on DEAE-cellulose, Sepharose 6B, hydroxyapatite, glutamate dehydrogenase antibody affinity chromatography and column chromatography on Sephacryl S-200 [Brevibacterium] flavum

Reaction

EC Number Reaction Comment Organism Reaction ID
1.4.1.13 2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH + H+ hexa-uni ping pong mechanism [Brevibacterium] flavum
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.13 30.5
-
-
 [Brevibacterium] flavum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.13 L-glutamine + 2-oxoglutarate + NADPH + H+ highly specific [Brevibacterium] flavum L-glutamate + NADP+
-
 ?
1.4.1.13 L-glutamine + 2-oxoglutarate + NADPH + H+ glyoxylate shows 3% reactivity compared with alpha-ketoglutarate [Brevibacterium] flavum L-glutamate + NADP+
-
 ?
1.4.1.13 L-glutamine + 2-oxoglutarate + NADPH + H+ ammonia does not replace L-glutamine as amino donor [Brevibacterium] flavum L-glutamate + NADP+
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.13 7
-
-
 [Brevibacterium] flavum

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.13 flavin enzyme contains 2 mol of FMN and 2 mol of FAD [Brevibacterium] flavum
1.4.1.13 NADH no activity with NADH [Brevibacterium] flavum
1.4.1.13 NADPH
-
 [Brevibacterium] flavum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.4.1.13 0.01
-
 methionine sulfone competitive inhibitor with respect to glutamine [Brevibacterium] flavum
1.4.1.13 0.041
-
 methionine sulfone uncompetitive inhibitor with respect to alpha-ketoglutarate [Brevibacterium] flavum
1.4.1.13 0.045
-
 methionine sulfone uncompetitive inhibitor with respect to NADPH [Brevibacterium] flavum
1.4.1.13 0.25
-
 NADP competitive inhibitor vs. NADPH [Brevibacterium] flavum
1.4.1.13 0.69
-
 NADP uncompetitive inhibitor vs. alpha-ketoglutarate [Brevibacterium] flavum
1.4.1.13 0.8
-
 oxalylglycine competitive inhibitor with respect to alpha-ketoglutarate [Brevibacterium] flavum
1.4.1.13 1.13
-
 NADP uncompetitive inhibitor vs. glutamine [Brevibacterium] flavum
1.4.1.13 2.1
-
 2'-adenylic acid competitive inhibitor with respect to NADPH [Brevibacterium] flavum
1.4.1.13 17.1
-
 2'-adenylic acid uncompetitive inhibitor with respect to glutamine [Brevibacterium] flavum
1.4.1.13 17.2
-
 2'-adenylic acid uncompetitive inhibitor with respect to alpha-keto-glutarate [Brevibacterium] flavum
1.4.1.13 19.3
-
 oxalylglycine uncompetitive inhibitor with respect to glutamine [Brevibacterium] flavum
1.4.1.13 20.6
-
 oxalylglycine uncompetitive inhibitor with respect to NADP [Brevibacterium] flavum
1.4.1.13 25
-
 glutamate competitive inhibitor with respect to glutamine [Brevibacterium] flavum
1.4.1.13 45
-
 glutamate competitive inhibitor with respect to alpha-ketoglutarate [Brevibacterium] flavum