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Literature summary extracted from

  • Samain, E.; Patil, D.S.; DerVartanian, D.V.; Albagnac, G.; LeGall, J.
    Isolation of succinate dehydrogenase from Desulfobulbus elongatus, a propionate oxidizing, sulfate reducing bacterium (1987), FEBS Lett., 216, 140-144.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.5.1 4-Chloromercuriphenyl sulfonate
-
 Desulfobulbus elongatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.5.1 0.0149
-
 fumarate
-
 Desulfobulbus elongatus
1.3.5.1 0.023
-
 succinate
-
 Desulfobulbus elongatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.5.1 membrane
-
 Desulfobulbus elongatus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.5.1 Fe 8 mol nonheme iron per mol of succinate dehydrogenase Desulfobulbus elongatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.5.1 118000
-
 calculation from subunit composition Desulfobulbus elongatus
1.3.5.1 170000
-
 sedimentation equilibrium centrifugation of preparation containing Triton X-100 Desulfobulbus elongatus

Organism

EC Number Organism UniProt Comment Textmining
1.3.5.1 Desulfobulbus elongatus
-
 succinate dehydrogenase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.5.1 using solubilization with Triton X-100 and column chromatography on DEAE and Ultrogel ACA Desulfobulbus elongatus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.5.1 additional information
-
-
 Desulfobulbus elongatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.5.1 fumarate + reduced 2,6-dichlorophenolindophenol
-
 Desulfobulbus elongatus succinate + 2,6-dichlorophenolindophenol
-
 r
1.3.5.1 succinate + 2,6-dichlorophenolindophenol
-
 Desulfobulbus elongatus fumarate + reduced 2,6-dichlorophenolindophenol
-
 r

Subunits

EC Number Subunits Comment Organism
1.3.5.1 trimer 1 * 65000-68700, Fp-subunit, FAD-binding, 1 * 27500-28000, Ip-subunit, Fe-S cluster containing, 1 * 19000-22000, membrane binding and cytochrome b containing subunit, SDS-PAGE Desulfobulbus elongatus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.5.1 cytochrome b 1 mol per mol of succinate dehydrogenase Desulfobulbus elongatus
1.3.5.1 FAD 1 mol flavin per mol succinate dehydrogenase Desulfobulbus elongatus