EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.3.3.6 | phenol | low concentration, magnitude of activation depends on the nature of the acyl-CoA substrate | Yarrowia lipolytica |
EC Number | Application | Comment | Organism |
---|---|---|---|
1.3.3.6 | biotechnology | potential depolluting agent by degradation of oils | Yarrowia lipolytica |
1.3.3.6 | biotechnology | several biotechnological applications: production of metabolites, such as citrate, secretion of proteins, degradation of fatty acids | Yarrowia lipolytica |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.3.6 | expression of His-tagged enzyme in Escherichia coli | Yarrowia lipolytica |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.3.3.6 | phenol | high concentration, magnitude of inhibition depends on the nature of the acyl-CoA substrate | Yarrowia lipolytica |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.3.6 | 0.002 | - |
decanoyl-CoA | recombinant enzyme | Yarrowia lipolytica | |
1.3.3.6 | 0.067 | - |
hexanoyl-CoA | recombinant enzyme | Yarrowia lipolytica |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.3.3.6 | peroxisome | - |
Yarrowia lipolytica | 5777 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.3.6 | acyl-CoA + O2 | Yarrowia lipolytica | involved in beta-oxidation of fatty acids in peroxisomes and glyoxysomes, respectively | trans-2,3-dehydroacyl-CoA + H2O2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.3.6 | Yarrowia lipolytica | - |
isoform AOX3 | - |
1.3.3.6 | Yarrowia lipolytica | - |
i.e. Yarrowia lipolytica | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.3.3.6 | recombinant from Escherichia coli, His-tagged | Yarrowia lipolytica |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.3.3.6 | 1.95 | - |
recombinant enzyme, purified | Yarrowia lipolytica |
EC Number | Storage Stability | Organism |
---|---|---|
1.3.3.6 | -30°C, 35% glycerol (v/v), at least 1 month | Yarrowia lipolytica |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.3.6 | acyl-CoA + O2 | medium-chain-length specific | Yarrowia lipolytica | trans-2,3-dehydroacyl-CoA + H2O2 | - |
? | |
1.3.3.6 | acyl-CoA + O2 | involved in beta-oxidation of fatty acids in peroxisomes and glyoxysomes, respectively | Yarrowia lipolytica | trans-2,3-dehydroacyl-CoA + H2O2 | - |
? | |
1.3.3.6 | decanoyl-CoA + O2 | - |
Yarrowia lipolytica | trans-2-decenoyl-CoA + H2O2 | - |
? | |
1.3.3.6 | furylpropionyl-CoA + O2 | also oxidizes aromatic/heterocyclic ring-substituted chromogenic substrates | Yarrowia lipolytica | furylacryloyl-CoA + H2O2 | - |
? | |
1.3.3.6 | hexanoyl-CoA + O2 | recombinant enzyme | Yarrowia lipolytica | (2E)-hex-2-enoyl-CoA + H2O2 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.3.6 | AOX3 | - |
Yarrowia lipolytica |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.3.3.6 | 28 | 38 | recombinant enzyme | Yarrowia lipolytica |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.3.3.6 | 5 | 55 | recombinant enzyme, at 5°C, 21% activity, at 55°C, 78% activity | Yarrowia lipolytica |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.3.6 | 1.53 | - |
decanoyl-CoA | recombinant enzyme | Yarrowia lipolytica | |
1.3.3.6 | 8.59 | - |
hexanoyl-CoA | recombinant enzyme | Yarrowia lipolytica |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.3.3.6 | 7.4 | - |
recombinant enzyme | Yarrowia lipolytica |