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Literature summary extracted from

  • Pfitzner A.; Stöckigt, J.
    Partial purification and characterization of geissoschizzine dehydrogenase from suspension cultures of Catharanthus roseus (1982), Phytochemistry, 21, 1585-1588.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.36 0.000077
-
 NADP+
-
 Catharanthus roseus
1.3.1.36 0.083
-
 Geissoschizine
-
 Catharanthus roseus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.36 geissoschizine + NADP+ Catharanthus roseus involved in interconversion of heteroyohimbine alkaloids, the enzyme channels geissoschizine by a side reaction into the main pathway 4,21-dehydrogeissoschizine + NADPH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.36 Catharanthus roseus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.36 partial Catharanthus roseus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.3.1.36 geissoschizine + NADP+ = 4,21-didehydrogeissoschizine + NADPH removal of 21alpha-hydrogen takes place from the less hindered site Catharanthus roseus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.36 cell suspension culture
-
 Catharanthus roseus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.36 0.000168
-
-
 Catharanthus roseus

Storage Stability

EC Number Storage Stability Organism
1.3.1.36 0°C, 14 days Catharanthus roseus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.36 geissoschizine + NADP+ structurally related indole alkaloids are not dehydrogenated Catharanthus roseus 4,21-dehydrogeissoschizine + NADPH
-
 ?
1.3.1.36 geissoschizine + NADP+ involved in interconversion of heteroyohimbine alkaloids, the enzyme channels geissoschizine by a side reaction into the main pathway Catharanthus roseus 4,21-dehydrogeissoschizine + NADPH
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.36 30
-
 assay at Catharanthus roseus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.36 7.6
-
-
 Catharanthus roseus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.3.1.36 5 8.5
-
 Catharanthus roseus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.36 NADP+ NAD+, FAD or FMN cannot act as cofactors Catharanthus roseus