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Literature summary extracted from
- Studies on the metabolism of kynurenic acid (1963), J. Biol. Chem., 238, 283-293.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.14.99.2 | 2-mercaptoethanol stabilizes activity | Pseudomonas fluorescens |
| 1.14.99.2 | cysteine stabilizes activity | Pseudomonas fluorescens |
| 1.14.99.2 | GSH stabilizes activity | Pseudomonas fluorescens |
| 1.14.99.2 | precipitation of supernatant with ammonium sulfate in the absence of cysteine, complete loss of activity | Pseudomonas fluorescens |
| 1.14.99.2 | thioglycolate stabilizes activity | Pseudomonas fluorescens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.99.2 | Ag+ | - |
Pseudomonas fluorescens |  |
| 1.14.99.2 | Cu2+ | - |
Pseudomonas fluorescens | |
| 1.14.99.2 | Hg2+ | - |
Pseudomonas fluorescens | |
| 1.14.99.2 | iodoacetate | inhibition reversed by addition of GSH or cysteine | Pseudomonas fluorescens | |
| 1.14.99.2 | Kynurenate | competitive inhibition at 0.2 mM | Pseudomonas fluorescens | |
| 1.14.99.2 | p-chloromercuribenzoate | inhibition reversed by addition of GSH or cysteine | Pseudomonas fluorescens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.99.2 | 0.008 | - |
Kynurenate | - |
Pseudomonas fluorescens | |
| 1.14.99.2 | 0.04 | - |
NADH | - |
Pseudomonas fluorescens | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.99.2 | kynurenate + NADH + O2 | Pseudomonas fluorescens | - |
7,8-dihydro-7,8-dihydroxykynurenate + NAD+ | - |
? | |
| 1.14.99.2 | kynurenate + NADPH + O2 | Pseudomonas fluorescens | - |
7,8-dihydro-7,8-dihydroxykynurenate + NADP+ | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.3.1.18 | Pseudomonas fluorescens | - |
ATCC 11299b | - |
| 1.14.99.2 | Pseudomonas fluorescens | - |
ATCC 11299b | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.3.1.18 | partial | Pseudomonas fluorescens |
| 1.14.99.2 | partial | Pseudomonas fluorescens |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.14.99.2 | -10°C, ammonium sulfate fractions, loss of activity after a few days | Pseudomonas fluorescens |
| 1.14.99.2 | -10°C, supernatant after high speed centrifugation of crude extract, several months, no loss in activity | Pseudomonas fluorescens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.3.1.18 | 7,8-dihydro-7,8-dihydroxykynurenate + NAD+ | - |
Pseudomonas fluorescens | 7,8-dihydroxykynurenate + NADH | - |
ir | |
| 1.14.99.2 | kynurenate + NADH + O2 | - |
Pseudomonas fluorescens | 7,8-dihydro-7,8-dihydroxykynurenate + NAD+ | - |
? | |
| 1.14.99.2 | kynurenate + NADPH + O2 | - |
Pseudomonas fluorescens | 7,8-dihydro-7,8-dihydroxykynurenate + NADP+ | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.3.1.18 | 23 | - |
assay at | Pseudomonas fluorescens |
| 1.14.99.2 | 23 | - |
assay at | Pseudomonas fluorescens |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.99.2 | 60 | - |
5 min, complete inactivation | Pseudomonas fluorescens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.99.2 | 7 | - |
- |
Pseudomonas fluorescens |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.99.2 | 9 | - |
stable in presence 10 mM cysteine | Pseudomonas fluorescens |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.3.1.18 | NAD+ | - |
Pseudomonas fluorescens | |
| 1.14.99.2 | NADH | - |
Pseudomonas fluorescens | |
| 1.14.99.2 | NADPH | - |
Pseudomonas fluorescens | |
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