EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.3.1.15 | soluble | - |
Pseudomonas sp. | - |
- |
1.3.98.1 | particle-bound | - |
Pseudomonas sp. | - |
- |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.15 | orotate + NADPH | Pseudomonas sp. | degradative enzyme | (S)-dihydroorotate + NADP+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.1.15 | Pseudomonas sp. | - |
pseudomonad isolated from lake bank mud, capable of growing on salt medium with glucose, aspartate, glycerol or orotate as carbon source | - |
1.3.98.1 | Pseudomonas sp. | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.3.98.1 | centrifugation after cell disruption, separation from the NADP-linked dihydroorotic dehydrogenase | Pseudomonas sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.3.1.15 | 0.19 | - |
enzyme activity in cells grown on orotate | Pseudomonas sp. |
1.3.98.1 | 0.032 | - |
- |
Pseudomonas sp. |
EC Number | Storage Stability | Organism |
---|---|---|
1.3.1.15 | -20°C, 4 months, complete loss of activity | Pseudomonas sp. |
1.3.98.1 | -20°C, 4 months | Pseudomonas sp. |
1.3.98.1 | 0°C, 1 week | Pseudomonas sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.15 | orotate + NADPH | enzyme is 20fold less active with NADH as cofactor | Pseudomonas sp. | (S)-dihydroorotate + NADP+ | - |
? | |
1.3.1.15 | orotate + NADPH | degradative enzyme | Pseudomonas sp. | (S)-dihydroorotate + NADP+ | - |
? | |
1.3.98.1 | (S)-dihydroorotate + O2 | ferricyanide as alternate electron acceptor, enzyme produced regardless of the carbon source | Pseudomonas sp. | orotate + H2O2 | - |
? |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.15 | NADPH | - |
Pseudomonas sp. |