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Literature summary extracted from

  • Maki, Y.; Yamamoto, S.; Nozaki, M.; Hayaishi, O.
    Studies on monooxygenases. II. Crystallization and some properties of imidazole acetate monooxygenase (1969), J. Biol. Chem., 244, 2942-2950.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.13.5
-
 Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.5 indoleacetate 30% inhibition at 10 mM Pseudomonas sp.
1.14.13.5 mercury compounds
-
 Pseudomonas sp.
1.14.13.5 nicotinate 65% inhibition at 20 mM Pseudomonas sp.
1.14.13.5 p-chloromercuribenzoate
-
 Pseudomonas sp.
1.14.13.5 phenylacetate 79% inhibition at 20 mM Pseudomonas sp.
1.14.13.5 silver compounds
-
 Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.5 0.002 0.01 NADH
-
 Pseudomonas sp.
1.14.13.5 0.01
-
 NADH spectrophotometric method Pseudomonas sp.
1.14.13.5 0.01
-
 Sodium diethyldithiocarbamate polarographic method Pseudomonas sp.
1.14.13.5 0.015
-
 NADH substrate: sodium diethyldithiocarbamate, polarographic method Pseudomonas sp.
1.14.13.5 0.02
-
 O2 substrate: imidazoleacetate or sodium diethyldithiocarbamate, polarographic method Pseudomonas sp.
1.14.13.5 0.03
-
 NADH polarographic method Pseudomonas sp.
1.14.13.5 0.3
-
 imidazoleacetate spectrophotometric method Pseudomonas sp.
1.14.13.5 0.5
-
 imidazoleacetate polarographic method Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.5 87000 90000 sedimentation equilibrium, gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.5 4-imidazoleacetate + NADH + O2 Pseudomonas sp.
-
 5-hydroxy-4-imidazoleacetate + NAD+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.5 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.5 using protamine sulfate treatment, ammonium sulfate fractionation, chromatography on TEAE-cellulose column, calcium phosphate gel treatment and chromatography on DEAE-Sephadex column Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.5 25
-
-
 Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
1.14.13.5 -20°C, potassium phosphate buffer, pH 7.2, no loss of activity for at least 1 month Pseudomonas sp.
1.14.13.5 4°C, 5-100 mM potassium phosphate buffer, pH 7.2, almost full activity retained for several days Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.5 4-imidazoleacetate + NAD(P)H + O2 + H+ electrons can be trasferred from NADH to 2,6-dichloroindophenol in the presence of the enzyme Pseudomonas sp. 5-hydroxy-4-imidazoleacetate + NAD(P)+ + H2O
-
 ?
1.14.13.5 4-imidazoleacetate + NADH + O2
-
 Pseudomonas sp. 5-hydroxy-4-imidazoleacetate + NAD+ + H2O
-
 ?
1.14.13.5 imidazolelactate + NADH + O2 + H+
-
 Pseudomonas sp. 5-hydroxyimidazolelactate + NAD+ + H2O
-
 ?
1.14.13.5 imidazolepropionate + NAD(P)H + O2 + H+
-
 Pseudomonas sp. 5-hydroxyimidazolepropionate + NAD(P)+ + H2O
-
 ?
1.14.13.5 sodium diethyldithiocarbamate + NADH + O2
-
 Pseudomonas sp. ? + NAD+ + H2O reaction product not identified ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.13.5 20
-
 2% loss of activity, 0.1 M potassium phosphate, pH 7.2, 5 min Pseudomonas sp.
1.14.13.5 30
-
 50% loss of activity, 0.1 M potassium phosphate, pH 7.2, 5 min Pseudomonas sp.
1.14.13.5 40
-
 95% loss of activity, 0.1 M potassium phosphate, pH 7.2, 5 min Pseudomonas sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.13.5 37.7
-
 imidazoleacetate
-
 Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.5 9
-
-
 Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.5 FAD 1 mol per mol enzyme Pseudomonas sp.
1.14.13.5 FAD cannot be replaced by FMN or riboflavin Pseudomonas sp.
1.14.13.5 NADH
-
 Pseudomonas sp.
1.14.13.5 NADPH beta-NADPH is approximately 13% as active as beta-NADH Pseudomonas sp.
1.14.13.5 NADPH alpha-NADH cannot replace beta-NADH at 0.2 mM Pseudomonas sp.