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Literature summary extracted from

  • Hosokawa, K.; Stanier, R.Y.
    Crystallization and properties of p-hydroxybenzoate hydroxylase from Pseudomonas putida (1966), J. Biol. Chem., 241, 2453-2460.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.13.2
-
 Pseudomonas fluorescens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.2 3-hydroxybenzoate above 1 mM Pseudomonas fluorescens
1.14.13.2 4-Aminobenzoate above 1 mM Pseudomonas fluorescens
1.14.13.2 4-Fluorobenzoate above 1 mM Pseudomonas fluorescens
1.14.13.2 4-hydroxybenzoate above 1 mM Pseudomonas fluorescens
1.14.13.2 benzoate above 1 mM Pseudomonas fluorescens
1.14.13.2 Fe2+
-
 Pseudomonas fluorescens
1.14.13.2 protocatechuate above 1 mM Pseudomonas fluorescens
1.14.13.2 salicylate above 1 mM Pseudomonas fluorescens
1.14.13.2 Zn2+ 0.2 mM, reversed by 10 mM glutathione Pseudomonas fluorescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.2 0.0213
-
 4-hydroxybenzoate
-
 Pseudomonas fluorescens
1.14.13.2 0.0227
-
 NADPH reaction with 4-hydroxybenzoate Pseudomonas fluorescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.2 78000
-
 gel filtration Pseudomonas fluorescens
1.14.13.2 83600
-
 low speed sedimentation without reaching equilibrium Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.2 4-hydroxybenzoate + NADPH + O2 Pseudomonas fluorescens inducible enzyme ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.2 Pseudomonas fluorescens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.2 2,4-dihydroxybenzoate + NADPH + O2 1.5% of the activity with 4-hydroxybenzoate Pseudomonas fluorescens 2,3,4-trihydroxybenzoate + 2,4,5-trihydroxybenzoate + NADP+ + H2O
-
 ?
1.14.13.2 3-bromo-4-hydroxybenzoate + NADPH + O2 3.2% of the activity with 4-hydroxybenzoate Pseudomonas fluorescens ?
-
 ?
1.14.13.2 4-hydroxybenzoate + NADPH + O2
-
 Pseudomonas fluorescens protocatechuate + NADP+ + H2O
-
 ?
1.14.13.2 4-hydroxybenzoate + NADPH + O2 inducible enzyme Pseudomonas fluorescens ?
-
 ?
1.14.13.2 4-toluate + NADPH + O2 0.29% of the activity with 4-hydroxybenzoate Pseudomonas fluorescens ?
-
 ?
1.14.13.2 benzene sulfonate + NADPH + O2 0.34% of the activity with 4-hydroxybenzoate Pseudomonas fluorescens ?
-
 ?
1.14.13.2 additional information under anaerobic conditions, the enzyme can catalyze a reduction of FAD by NADPH provided that 4-hydroxybenzoate is present Pseudomonas fluorescens ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.13.2 36.7
-
 4-hydroxybenzoate
-
 Pseudomonas fluorescens

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.2 FAD contains approximately 0.8 mol FAD per mol of enzyme Pseudomonas fluorescens
1.14.13.2 NADPH no activity with NADH Pseudomonas fluorescens