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Literature summary extracted from

  • Uwajima, T.; Terada, O.
    Properties of crystalline 3alpha-hydroxysteroid dehydrogenase from Pseudomonas putida (1979), Agric. Biol. Chem., 43, 1521-1528.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.50
-
 Pseudomonas putida

General Stability

EC Number General Stability Organism
1.1.1.50 mercaptoethanol stabilizes Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.50 heavy metal ions
-
 Pseudomonas putida
1.1.1.50 p-chloromercuribenzoate
-
 Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.50 0.01 0.025 cholic acid
-
 Pseudomonas putida
1.1.1.50 0.87
-
 NAD+
-
 Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.50 45000
-
 sedimentation equilibrium centrifugation Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.50 Pseudomonas putida
-
-
-

Storage Stability

EC Number Storage Stability Organism
1.1.1.50 0-3°C, lyophilized, at least 6 months Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.50 3alpha-hydroxy-12-oxo-9,11-cholenic acid + NADP+
-
 Pseudomonas putida 3,12-dioxo-9,11-cholenic acid + NADPH
-
 ?
1.1.1.50 5alpha-dihydrotestosterone + NAD(P)H + H+ i.e. 5alpha-androstan-17beta-ol-3-one Pseudomonas putida 5alpha-androstan-3alpha,17beta-diol + NAD(P)+
-
 r
1.1.1.50 5alpha-dihydrotestosterone + NAD(P)H + H+ i.e. 5alpha-androstan-17beta-ol-3-one Pseudomonas putida (3beta,5alpha,17beta)-androstane-3,17-diol + NAD(P)+
-
 r
1.1.1.50 androsterone + NAD(P)+ i.e. 3alpha-hydroxy-5alpha-androstan-17-one Pseudomonas putida 5alpha-androstan-3,17-dione + NAD(P)H
-
 r
1.1.1.50 chenodeoxycholic acid + NAD(P)+ i.e. 3alpha,7alpha-dihydroxy-5beta-cholan-24-oic acid Pseudomonas putida 7alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NAD(P)H
-
 r
1.1.1.50 cholic acid + NAD(P)+ i.e. 3alpha,7alpha,12alpha-trihydroxy-5beta-cholan-24-oic acid Pseudomonas putida 7alpha,12alpha-dihydroxy-3-oxo-5beta-cholan-24-oic acid + NAD(P)H
-
 ?
1.1.1.50 deoxycholic acid + NAD(P)+ i.e. 3alpha,12alpha-dihydroxy-5beta-cholan-24-oic acid Pseudomonas putida 12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NAD(P)H + H+
-
 ?
1.1.1.50 etiocholan-3alpha-ol-17-one + NADP+
-
 Pseudomonas putida etiocholan-3,17-dione + NADPH
-
 ?
1.1.1.50 lithocholic acid + NADP+ i.e. 3alpha-hydroxy-5beta-cholan-24-oic acid Pseudomonas putida 3-oxo-5beta-cholan-24-oic acid + NADPH
-
 ?
1.1.1.50 additional information acts faster on A/B cis hydroxysteroids than on A/B trans hydroxysteroids Pseudomonas putida ?
-
 ?
1.1.1.50 additional information absolutely specific for 3alpha-hydroxy group of C19, C21 and C24 steroids Pseudomonas putida ?
-
 ?
1.1.1.50 taurocholic acid + NAD(P)+ i.e. 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl taurine Pseudomonas putida 2[[7alpha,12alpha-dihydroxy-3,24-dioxo-5beta-cholan-24-yl]amino]ethane sulfonic acid + NADPH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.50 monomer 1 * 45000, sedimentation equilibrium, presence of 2-mercaptoethanol Pseudomonas putida

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.50 40
-
 15 min stable in presence of mercaptoethanol, 10% loss of activity in absence of mercaptoethanol Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.50 7.9
-
 reduction of etiocholan-3,17-dione Pseudomonas putida

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.50 5 10.5 stable for 30 min at 30°C Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.50 additional information see also EC 1.1.1.213 for enzymes catalyzing the same reaction but with A-specificity concerning the cofactor, all enzymes without information of stereospecificity for cofactors are included in EC 1.1.1.50, but some of those may in fact belong to EC 1.1.1.213 Pseudomonas putida