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Literature summary extracted from

  • Clark, D.D.; Ensign, S.A.
    Characterization of the 2-[(R)-2-hydroxypropylthio]ethanesulfonate dehydrogenase from Xanthobacter Strain Py2: Product inhibition, pH dependence of kinetic parameters, site-directed mutagenesis, rapid equilibrium inhibition, and chemical modification (2002), Biochemistry, 41, 2727-2740.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.268 expression in Escherichia coli Xanthobacter sp.

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.268 K159A inactive enzyme Xanthobacter sp.
1.1.1.268 S142A greatly reduced activity Xanthobacter sp.
1.1.1.268 S142C inactive enzyme Xanthobacter sp.
1.1.1.268 Y155E inactive enzyme Xanthobacter sp.
1.1.1.268 Y155F inactive enzyme Xanthobacter sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.268 2-(2-methyl-2-hydroxypropylthio)ethanesulfonate
-
 Xanthobacter sp.
1.1.1.268 2-(S)-hydroxypropyl-CoM
-
 Xanthobacter sp.
1.1.1.268 2-oxopropyl-CoM mixed-type inhibitor with respect to 2-(R)-hydroxypropyl-CoM Xanthobacter sp.
1.1.1.268 ethanesulfonate
-
 Xanthobacter sp.
1.1.1.268 mercaptoethanesulfonate
-
 Xanthobacter sp.
1.1.1.268 methanesulfonate
-
 Xanthobacter sp.
1.1.1.268 NADH mixed-type inhibitor with respect to 2-(R)-hydroxypropyl-CoM, competitive inhibition with respect to 2-oxopropyl-CoM Xanthobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.268 63900
-
 gel filtration Xanthobacter sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.268 2-(R)-hydroxypropyl-CoM + NAD+ Xanthobacter sp.
-
 2-oxopropyl-CoM + NADH
-
 r
1.1.1.268 2-(R)-hydroxypropyl-CoM + NAD+ Xanthobacter sp. Py2
-
 2-oxopropyl-CoM + NADH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.268 Xanthobacter sp.
-
-
-
1.1.1.268 Xanthobacter sp. Py2
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.268 to homogeneity, recombinant enzyme Xanthobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.268 2-(2-hydroxyethylthio)ethanesulfonate + NAD+
-
 Xanthobacter sp. 2-(2-oxoethylthio)ethanesulfonate + NADH
-
 ?
1.1.1.268 2-(2-hydroxyethylthio)ethanesulfonate + NAD+
-
 Xanthobacter sp. Py2 2-(2-oxoethylthio)ethanesulfonate + NADH
-
 ?
1.1.1.268 2-(R)-hydroxypropyl-CoM + NAD+
-
 Xanthobacter sp. 2-oxopropyl-CoM + NADH
-
 r
1.1.1.268 2-(R)-hydroxypropyl-CoM + NAD+
-
 Xanthobacter sp. Py2 2-oxopropyl-CoM + NADH
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.268 dimer
-
 Xanthobacter sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.268 0.29
-
 2-(2-hydroxyethylthio)ethanesulfonate
-
 Xanthobacter sp.
1.1.1.268 24.5
-
 2-oxopropyl-CoM
-
 Xanthobacter sp.
1.1.1.268 25.8
-
 2-(R)-hydroxypropyl-CoM
-
 Xanthobacter sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.268 2-mercaptoethanesulfonate
-
 Xanthobacter sp.
1.1.1.268 NAD+
-
 Xanthobacter sp.
1.1.1.268 NADH
-
 Xanthobacter sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.268 0.156
-
 2-(S)-hydroxypropyl-CoM competitive inhibition with respect to 2-(R)-hydroxypropyl-CoM Xanthobacter sp.
1.1.1.268 0.406
-
 2-(2-methyl-2-hydroxypropylthio)ethanesulfonate competitive inhibition with respect to 2-(R)-hydroxypropyl-CoM Xanthobacter sp.
1.1.1.268 2.25
-
 mercaptoethanesulfonate mixed-type inhibition with respect to 2-(R)-hydroxypropyl-CoM Xanthobacter sp.
1.1.1.268 26.7
-
 ethanesulfonate mixed-type inhibition with respect to 2-(R)-hydroxypropyl-CoM Xanthobacter sp.
1.1.1.268 147.8
-
 methanesulfonate mixed-type inhibition with respect to 2-(R)-hydroxypropyl-CoM Xanthobacter sp.