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Literature summary extracted from
- A type I DNA topoisomerase from Trypanosoma cruzi (1983), Eur. J. Biochem., 134, 479-484.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.6.2.1 | spermidine | enhances relaxation activity | Trypanosoma cruzi |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.6.2.1 | Berenil | - |
Trypanosoma cruzi | |
| 5.6.2.1 | Ethidium bromide | - |
Trypanosoma cruzi | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 5.6.2.1 | nucleus | - |
Trypanosoma cruzi | 5634 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.6.2.1 | Mg2+ | enhances relaxation activity | Trypanosoma cruzi | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.6.2.1 | 65000 | - |
1 * 65000, SDS-PAGE | Trypanosoma cruzi |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.6.2.1 | Trypanosoma cruzi | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.6.2.1 | - |
Trypanosoma cruzi |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.6.2.1 | additional information | - |
- |
Trypanosoma cruzi |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.6.2.1 | supercoiled DNA | relaxation | Trypanosoma cruzi | relaxed closed circular DNA | - |
? | |
| 5.6.2.1 | supercoiled DNA | positive and negative supercoiled DNA | Trypanosoma cruzi | relaxed closed circular DNA | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.6.2.1 | monomer | 1 * 65000, SDS-PAGE | Trypanosoma cruzi |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.6.2.1 | 30 | 37 | - |
Trypanosoma cruzi |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.6.2.1 | 10 | 40 | active in this range | Trypanosoma cruzi |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.6.2.1 | 5.4 | 8.5 | active in this range | Trypanosoma cruzi |
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Release 2023.1 (January 2023)
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