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Literature summary extracted from

  • Suhar, A.; Marks, N.
    Purification and properties of brain cathepsin B. Evidence for cleavage of pituitary lipotropins (1979), Eur. J. Biochem., 101, 23-30.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.1 2-mercaptoethanol activates Bos taurus
3.4.22.1 cysteine activates Bos taurus
3.4.22.1 dithiothreitol activates Bos taurus
3.4.22.1 EDTA activates Bos taurus
3.4.22.1 thiol compounds absolute requirement Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.1 antipain
-
 Bos taurus
3.4.22.1 chymostatin 80% inhibition at 6 microM Bos taurus
3.4.22.1 Hg2+
-
 Bos taurus
3.4.22.1 iodoacetic acid
-
 Bos taurus
3.4.22.1 leupeptin
-
 Bos taurus
3.4.22.1 Zn2+
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.1 0.53
-
 benzoyl-DL-Arg-beta-naphthylamide
-
 Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.1 27500
-
 gel filtration, SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.1 Proteins + H2O Bos taurus turnover of intracellular proteins ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.1 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.1
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.1 cerebral cortex
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.1 benzoyl-DL-Arg-beta-naphthylamide + H2O
-
 Bos taurus benzoyl-DL-Arg + beta-naphthylamine
-
 ?
3.4.22.1 beta-lipotropin + H2O
-
 Bos taurus hydrolyzed beta-lipotropin
-
 ?
3.4.22.1 histones + H2O
-
 Bos taurus hydrolyzed histones
-
 ?
3.4.22.1 Proteins + H2O turnover of intracellular proteins Bos taurus ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.1 0.075
-
 benzoyl-DL-Arg-beta-naphthylamide
-
 Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.1 6.5
-
-
 Bos taurus