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Literature summary extracted from
- Purification and partial characterization of a prolyl-dipeptidyl aminopeptidase from Lactobacillus helveticus CNRZ 32 (1990), Appl. Environ. Microbiol., 56, 381-388.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.14.5 | 1,10-phenanthroline | 1.0 mM, 14 mM 40°C, partial | Lactobacillus helveticus |  |
| 3.4.14.5 | Cu2+ | 0.1-2.5 mM | Lactobacillus helveticus | |
| 3.4.14.5 | diisopropyl fluorophosphate | 1.0 mM, 2 h, 40°C, complete inhibition | Lactobacillus helveticus | |
| 3.4.14.5 | EDTA | 1.0 mM, 40°C, 20 h, partial | Lactobacillus helveticus | |
| 3.4.14.5 | Hg2+ | 0.1-2.5 mM | Lactobacillus helveticus | |
| 3.4.14.5 | iodoacetate | 1.0 mM, partial | Lactobacillus helveticus | |
| 3.4.14.5 | NEM | 1.0 mM, partial | Lactobacillus helveticus | |
| 3.4.14.5 | PCMB | 1.0 mM, 15 min, 40°C, complete inhibition | Lactobacillus helveticus | |
| 3.4.14.5 | phenylmethylsulfonyl fluoride | 1.0 mM, partial | Lactobacillus helveticus | |
| 3.4.14.5 | Zn2+ | 0.1-2.5 mM | Lactobacillus helveticus | |
| 3.4.14.11 | 1,10-phenanthroline | - |
Lactobacillus helveticus | |
| 3.4.14.11 | Cu2+ | - |
Lactobacillus helveticus | |
| 3.4.14.11 | diisopropylfluorophosphate | - |
Lactobacillus helveticus | |
| 3.4.14.11 | EDTA | - |
Lactobacillus helveticus | |
| 3.4.14.11 | Hg2+ | - |
Lactobacillus helveticus | |
| 3.4.14.11 | iodoacetate | - |
Lactobacillus helveticus | |
| 3.4.14.11 | additional information | not: DTT, 1.0-19 mM | Lactobacillus helveticus | |
| 3.4.14.11 | NEM | - |
Lactobacillus helveticus | |
| 3.4.14.11 | PCMB | - |
Lactobacillus helveticus | |
| 3.4.14.11 | phenylmethylsulfonyl fluoride | - |
Lactobacillus helveticus | |
| 3.4.14.11 | Zn2+ | - |
Lactobacillus helveticus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.14.5 | Ca2+ | 0.1-2.5 mM, activates | Lactobacillus helveticus | |
| 3.4.14.5 | K+ | 10-50 mM, activates | Lactobacillus helveticus | |
| 3.4.14.5 | Mg2+ | 2.5 mM, activates | Lactobacillus helveticus | |
| 3.4.14.5 | Na+ | 10-50 mM, activates | Lactobacillus helveticus | |
| 3.4.14.11 | Ca2+ | 0.1-2.5 mM, 40°C, 15 min, activation | Lactobacillus helveticus | |
| 3.4.14.11 | K+ | 10-50 mM, 40°C, 15 min, activation | Lactobacillus helveticus | |
| 3.4.14.11 | Mg2+ | 2.5 mM, 40°C, 15 min, activation | Lactobacillus helveticus | |
| 3.4.14.11 | Na+ | 10-50 mM, 40°C, 15 min, activation | Lactobacillus helveticus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.14.5 | 72000 | - |
non-denaturing PAGE | Lactobacillus helveticus |
| 3.4.14.11 | 72000 | - |
x * 72000, native PAGE and SDS-PAGE | Lactobacillus helveticus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.14.5 | Lactobacillus helveticus | - |
CNRZ 32 | - |
| 3.4.14.5 | Lactobacillus helveticus CNRZ 32 | - |
CNRZ 32 | - |
| 3.4.14.11 | Lactobacillus helveticus | - |
CNRZ 32 | - |
| 3.4.14.11 | Lactobacillus helveticus CNRZ 32 | - |
CNRZ 32 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.14.5 | - |
Lactobacillus helveticus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.14.5 | additional information | - |
- |
Lactobacillus helveticus |
| 3.4.14.11 | additional information | - |
- |
Lactobacillus helveticus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.14.5 | Ala-Ala-4-nitroanilide + H2O | 2% of the activity with Gly-Pro-4-nitroanilide | Lactobacillus helveticus | Ala-Ala + 4-nitroaniline | - |
? | |
| 3.4.14.5 | Ala-Ala-4-nitroanilide + H2O | 2% of the activity with Gly-Pro-4-nitroanilide | Lactobacillus helveticus CNRZ 32 | Ala-Ala + 4-nitroaniline | - |
? | |
| 3.4.14.5 | Ala-Pro-Gly + H2O | 11% of the activity with Gly-Pro-4-nitroanilide | Lactobacillus helveticus | Ala-Pro + Gly | - |
? | |
| 3.4.14.5 | Ala-Pro-Gly + H2O | 11% of the activity with Gly-Pro-4-nitroanilide | Lactobacillus helveticus CNRZ 32 | Ala-Pro + Gly | - |
? | |
| 3.4.14.5 | Arg-Pro-4-nitroanilide + H2O | 130% of the activity with Gly-Pro-4-nitroanilide | Lactobacillus helveticus | Arg-Pro + 4-nitroaniline | - |
? | |
| 3.4.14.5 | Arg-Pro-4-nitroanilide + H2O | 130% of the activity with Gly-Pro-4-nitroanilide | Lactobacillus helveticus CNRZ 32 | Arg-Pro + 4-nitroaniline | - |
? | |
| 3.4.14.5 | Gly-Pro-4-nitroanilide + H2O | - |
Lactobacillus helveticus | Gly-Pro + 4-nitroaniline | - |
? | |
| 3.4.14.5 | Gly-Pro-4-nitroanilide + H2O | - |
Lactobacillus helveticus CNRZ 32 | Gly-Pro + 4-nitroaniline | - |
? | |
| 3.4.14.11 | Ala-Ala 4-nitroanilide + H2O | 2% of the activity with Gly-Pro 4-nitroanilide | Lactobacillus helveticus | Ala-Ala + 4-nitroaniline | - |
? | |
| 3.4.14.11 | Ala-Pro-Gly + H2O | 11% of the activity with Gly-Pro 4-nitroanilide | Lactobacillus helveticus | Ala-Pro + Gly | - |
? | |
| 3.4.14.11 | Arg-Pro 4-nitroanilide + H2O | 130% of the activity with Gly-Pro 4-nitroanilide | Lactobacillus helveticus | Arg-Pro + 4-nitroaniline | - |
? | |
| 3.4.14.11 | Gly-Pro 4-nitroanilide + H2O | - |
Lactobacillus helveticus | Gly-Pro + 4-nitroaniline | - |
? | |
| 3.4.14.11 | additional information | no activity with Ala-Leu-Ala | Lactobacillus helveticus | ? | - |
? | |
| 3.4.14.11 | additional information | no activity with Ala-Leu-Ala | Lactobacillus helveticus CNRZ 32 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.14.5 | monomer | 1 * 72000, SDS-PAGE | Lactobacillus helveticus |
| 3.4.14.11 | ? | x * 72000, native PAGE and SDS-PAGE | Lactobacillus helveticus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.14.5 | 40 | - |
- |
Lactobacillus helveticus |
| 3.4.14.11 | 40 | - |
- |
Lactobacillus helveticus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.14.5 | 5 | 60 | about 40% of maximal activity at 5°C and at 60°C | Lactobacillus helveticus |
| 3.4.14.11 | 10 | 65 | 10°C: about 55% of activity maximum, 65°C: 34% of activity maximum | Lactobacillus helveticus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.14.5 | 7 | - |
hydrolysis of Gly-Pro-p-nitroanilide | Lactobacillus helveticus |
| 3.4.14.11 | 7 | - |
- |
Lactobacillus helveticus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.14.5 | 5 | 8.5 | pH 5.0: about 50% of maximal activity, pH 8.5: about 60% of maximal activity | Lactobacillus helveticus |
| 3.4.14.11 | 5 | 9 | pH 5: about 50% of maximal activity, pH 9: about 60% of maximal activity | Lactobacillus helveticus |
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