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Literature summary extracted from

  • Aranishi, F.; Hara, K.; Osatomi, K.; Ishihara, T.
    Purification and characterization of cathepsin B from hepatopancreas of carp Cyprinus carpio (1997), Comp. Biochem. Physiol. B, 117, 579-587.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.1 2-mercaptoethanol activates Cyprinus carpio
3.4.22.1 cysteine activates Cyprinus carpio
3.4.22.1 EDTA activates Cyprinus carpio

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.1 5,5'-dithiobis-2-nitrobenzoic acid
-
 Cyprinus carpio
3.4.22.1 antipain
-
 Cyprinus carpio
3.4.22.1 Cd2+
-
 Cyprinus carpio
3.4.22.1 chymostatin
-
 Cyprinus carpio
3.4.22.1 Cu2+
-
 Cyprinus carpio
3.4.22.1 Hg2+
-
 Cyprinus carpio
3.4.22.1 L-3-carboxy-2,3-trans-epoxypropionyl-leucylamido-(4-guanidino)butane E-64 Cyprinus carpio
3.4.22.1 leupeptin
-
 Cyprinus carpio
3.4.22.1 N-tosyl-lysyl chloromethylketone
-
 Cyprinus carpio
3.4.22.1 N-tosyl-phenylalanine chloromethylketone
-
 Cyprinus carpio
3.4.22.1 p-chloromercuribenzoate
-
 Cyprinus carpio
3.4.22.1 Zn2+
-
 Cyprinus carpio

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.1 0.093
-
 benzyloxycarbonyl-Arg-Arg-4-methylcoumaryl-7-amide
-
 Cyprinus carpio
3.4.22.1 0.129
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumaryl-7-amide
-
 Cyprinus carpio

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.1 29000 30000 gel filtration Cyprinus carpio
3.4.22.1 29000 30000 native PAGE Cyprinus carpio

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.1 Proteins + H2O Cyprinus carpio
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.1 Cyprinus carpio
-
 carp
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.1
-
 Cyprinus carpio

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.1 hepatopancreas
-
 Cyprinus carpio
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.1 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide + H2O
-
 Cyprinus carpio benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.1 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin-7-amide
-
 Cyprinus carpio benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.1 Proteins + H2O
-
 Cyprinus carpio ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.1 45
-
-
 Cyprinus carpio

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.22.1 30 55 greater activity than 50% of maximum Cyprinus carpio

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.1 8.97
-
 benzyloxycarbonyl-Arg-Arg-4-methylcoumaryl-7-amide
-
 Cyprinus carpio
3.4.22.1 34
-
 benzyloxycarbonyl-Phe-Arg-4-methylcoumaryl-7-amide
-
 Cyprinus carpio

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.1 6
-
-
 Cyprinus carpio

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.22.1 5.5 6.5 stable Cyprinus carpio