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Literature summary extracted from

  • Asano, Y.; Nakazawa, A.; Endo, K.
    Novel phenylalanine dehydrogenases from Sporosarcina ureae and Bacillus sphaericus. Purification and characterization (1987), J. Biol. Chem., 262, 10346-10354.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.4.1.20
-
 Lysinibacillus sphaericus
1.4.1.20
-
 Sporosarcina ureae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.20 5,5'-dithio-bis(2-nitrobenzoic acid) 0.17 mM: 26% inhibition Lysinibacillus sphaericus
1.4.1.20 5,5'-dithio-bis(2-nitrobenzoic acid) 0.17 mM, complete inhibition Sporosarcina ureae
1.4.1.20 AgNO3 1 mM, complete inhibition Lysinibacillus sphaericus
1.4.1.20 AgNO3 1 mM, complete inhibition Sporosarcina ureae
1.4.1.20 D-Leu 10 mM, 17% inhibition Lysinibacillus sphaericus
1.4.1.20 D-Leu 10 mM, 31% inhibition Sporosarcina ureae
1.4.1.20 D-Met 10 mM, 63% inhibition Lysinibacillus sphaericus
1.4.1.20 D-Met 10 mM, 25% inhibition Sporosarcina ureae
1.4.1.20 D-norleucine 10 mM, 52% inhibition Lysinibacillus sphaericus
1.4.1.20 D-norleucine 10 mM, 34% inhibition Sporosarcina ureae
1.4.1.20 D-Phe 10 mM, 89% inhibition Lysinibacillus sphaericus
1.4.1.20 D-Phe 10 mM, 77% inhibition Sporosarcina ureae
1.4.1.20 D-Trp 5 mM, 83% inhibition Lysinibacillus sphaericus
1.4.1.20 D-Trp 5 mM, 65% inhibition Sporosarcina ureae
1.4.1.20 D-Tyr 1 mM, 29% inhibition Lysinibacillus sphaericus
1.4.1.20 D-Val 10 mM, 11% inhibition Lysinibacillus sphaericus
1.4.1.20 HgCl2 0.01 mM, 62% inhibition Lysinibacillus sphaericus
1.4.1.20 HgCl2 0.01 M, complete inhibition Sporosarcina ureae
1.4.1.20 L-Cys 10 mM, 41% inhibition Lysinibacillus sphaericus
1.4.1.20 L-Ile 10 mM, 75% inhibition Lysinibacillus sphaericus
1.4.1.20 L-Leu 10 mM, 58% inhibition Lysinibacillus sphaericus
1.4.1.20 L-Leu 10 mM, 39% inhibition Sporosarcina ureae
1.4.1.20 L-Val 10 mM, 23% inhibition Lysinibacillus sphaericus
1.4.1.20 NEM 1 mM: 79% inhibition Sporosarcina ureae
1.4.1.20 PCMB 0.02 mM, complete inhibition Lysinibacillus sphaericus
1.4.1.20 PCMB 0.02 mM, complete inhibition Sporosarcina ureae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.20 0.025
-
 NADH
-
 Lysinibacillus sphaericus
1.4.1.20 0.072
-
 NADH
-
 Sporosarcina ureae
1.4.1.20 0.14
-
 NAD+
-
 Sporosarcina ureae
1.4.1.20 0.16
-
 phenylpyruvate
-
 Sporosarcina ureae
1.4.1.20 0.17
-
 NAD+
-
 Sporosarcina ureae
1.4.1.20 0.34
-
 4-hydroxyphenylpyruvate
-
 Sporosarcina ureae
1.4.1.20 0.4
-
 phenylpyruvate
-
 Sporosarcina ureae
1.4.1.20 2.44
-
 2-Oxohexanoate
-
 Sporosarcina ureae
1.4.1.20 78
-
 NH3
-
 Lysinibacillus sphaericus
1.4.1.20 85
-
 NH3
-
 Sporosarcina ureae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.20 305000
-
 equilibrium sedimentation Sporosarcina ureae
1.4.1.20 310000
-
 gel filtration Sporosarcina ureae
1.4.1.20 340000
-
 gel filtration, equilibrium sedimentation Lysinibacillus sphaericus

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.20 Lysinibacillus sphaericus
-
-
-
1.4.1.20 Sporosarcina ureae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.20
-
 Lysinibacillus sphaericus
1.4.1.20
-
 Sporosarcina ureae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.1.20 87.9
-
-
 Sporosarcina ureae
1.4.1.20 111
-
-
 Lysinibacillus sphaericus

Storage Stability

EC Number Storage Stability Organism
1.4.1.20 4°C, 0.01 M potassium phosphate buffer, pH 7.0, 0.1 mM EDTA, 5 mM 2-mercaptoethanol, stable for at least 2 years in crystalline form Lysinibacillus sphaericus
1.4.1.20 4°C, 0.01 M potassium phosphate buffer, pH 7.0, 0.1 mM EDTA, 5 mM 2-mercaptoethanol, stable for at least 2 years in crystalline form Sporosarcina ureae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.20 2-oxohexanoate + NH3 + NADH
-
 Sporosarcina ureae 2-aminohexanoate + H2O + NAD+
-
 ?
1.4.1.20 4-hydroxyphenylpyruvate + NH3 + NADH
-
 Lysinibacillus sphaericus L-Tyr + NAD+ + H2O
-
 ?
1.4.1.20 4-hydroxyphenylpyruvate + NH3 + NADH + H+
-
 Sporosarcina ureae L-tyrosine + NAD+ + H2O
-
 r
1.4.1.20 L-norleucine + H2O + NAD+
-
 Sporosarcina ureae 2-oxohexanoic acid + NADH + NH3
-
 ?
1.4.1.20 L-Phe + H2O + NAD+
-
 Lysinibacillus sphaericus phenylpyruvate + NH3 + NADH
-
 r
1.4.1.20 L-Phe + H2O + NAD+
-
 Sporosarcina ureae phenylpyruvate + NH3 + NADH
-
 r
1.4.1.20 L-phenylalanine methyl ester + H2O + NAD+
-
 Sporosarcina ureae phenylpyruvic acid methyl ester + NH3 + NADH
-
 ?
1.4.1.20 L-Tyr + H2O + NAD+
-
 Lysinibacillus sphaericus 4-hydroxyphenylpyruvate + NH3 + NADH
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.20 40
-
-
 Sporosarcina ureae
1.4.1.20 50
-
-
 Lysinibacillus sphaericus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.20 30
-
 pH 9.0, stable Sporosarcina ureae
1.4.1.20 40
-
 pH 9.0, 10 min, 25% loss of activity Sporosarcina ureae
1.4.1.20 50
-
 pH 9.0, 10 min, complete inactivation Sporosarcina ureae
1.4.1.20 55
-
 pH 9.0, 10 min, stable Lysinibacillus sphaericus
1.4.1.20 60
-
 pH 9.0, 10 min, most of the activity is lost Lysinibacillus sphaericus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.20 9
-
 reductive amination of phenylpyruvate Sporosarcina ureae
1.4.1.20 10.3
-
 reductive amination of phenylpyruvate Sporosarcina ureae
1.4.1.20 10.5
-
 oxidative deamination of L-Phe Sporosarcina ureae
1.4.1.20 11.3
-
 oxidative deamination of L-Phe Sporosarcina ureae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.1.20 4
-
 30°C, 1 h, less than 20% loss of activity Lysinibacillus sphaericus
1.4.1.20 9
-
 30°C, 1 h, stable Lysinibacillus sphaericus
1.4.1.20 9
-
 30°C, 1 h, stable Sporosarcina ureae
1.4.1.20 11.3
-
 30°C, 1 h, stable Lysinibacillus sphaericus

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.20 NAD+
-
 Lysinibacillus sphaericus
1.4.1.20 NAD+
-
 Sporosarcina ureae
1.4.1.20 NADH
-
 Lysinibacillus sphaericus
1.4.1.20 NADH
-
 Sporosarcina ureae