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Literature summary extracted from

  • Deyashiki, Y.; Tamada, Y.; Miyabe, Y.; Nakanishi, M.; Matsuura, K.; Hara, A.
    Expression and kinetic properties of a recombinant 3alpha-hydroxysteroid/dihydrodiol dehydrogenase isoenzyme of human liver (1995), J. Biochem., 118, 285-290.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.20 DD4, expression in Escherichia coli JM109 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.20 1-Indanone recombinant DD4 Homo sapiens
1.3.1.20 17beta-estradiol DD4, recombinant enzyme Homo sapiens
1.3.1.20 3',3'',5',5''-tetrabromophenolphthalein recombinant DD4 Homo sapiens
1.3.1.20 Betamethasone DD4, recombinant enzyme Homo sapiens
1.3.1.20 Bromophenol blue recombinant DD4 Homo sapiens
1.3.1.20 dexamethasone DD4, recombinant enzyme Homo sapiens
1.3.1.20 Flufenamic acid DD4, recombinant enzyme Homo sapiens
1.3.1.20 Hexestrol DD4, recombinant enzyme Homo sapiens
1.3.1.20 indomethacin recombinant DD4 Homo sapiens
1.3.1.20 Phenol red recombinant DD4 Homo sapiens
1.3.1.20 Phenolphthalein DD4, recombinant enzyme, inhibition mechanism Homo sapiens
1.3.1.20 phenolphthalein phosphate recombinant DD4 Homo sapiens
1.3.1.20 Thymolphthalein recombinant DD4 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.20 35000
-
 recombinant DD4, gel filtration Homo sapiens
1.3.1.20 36000
-
 DD4, native PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.20 Homo sapiens
-
 DD4
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.20 liver
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.20 2.23
-
 recombinant DD4, substrate (S)-indan-1-ol Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.20 (R)-indan-1-ol + NADP+
-
 Homo sapiens ?
-
 ?
1.3.1.20 (S)-indan-1-ol + NADP+
-
 Homo sapiens ?
-
 ?
1.3.1.20 5alpha-dihydrotestosterone + NADPH + H+
-
 Homo sapiens 5alpha-androstan-3alpha,17beta-diol + NADP+
-
 ?
1.3.1.20 5beta-androstan-3,17-dione + NADPH
-
 Homo sapiens 5beta-androstan-3alpha-ol-17-one + NADP+
-
 ?
1.3.1.20 5beta-androstan-3alpha,17beta-diol + NADP+
-
 Homo sapiens 5beta-androstan-3,17-dione + NADPH
-
 ?
1.3.1.20 5beta-dihydrocortisone + NADPH
-
 Homo sapiens ?
-
 ?
1.3.1.20 5beta-pregnan-3,20-diol + NADP+
-
 Homo sapiens 5beta-pregnan-20-ol-3-one + NADPH
-
 ?
1.3.1.20 5beta-pregnan-3,20-dione + NADPH
-
 Homo sapiens 3alpha-hydroxy-5beta-pregnan-20-one + NADP+
-
 ?
1.3.1.20 7alpha-hydroxy-5beta-cholestan-3-one + NADPH
-
 Homo sapiens ?
-
 ?
1.3.1.20 androsterone + NADPH
-
 Homo sapiens ?
-
 ?
1.3.1.20 cholic acid + NADP+
-
 Homo sapiens ?
-
 ?
1.3.1.20 lithocholic acid + NADP+
-
 Homo sapiens ?
-
 ?
1.3.1.20 pyridine-3-aldehyde + NADPH
-
 Homo sapiens ?
-
 ?
1.3.1.20 trans-1,2-dihydrobenzene-1,2-diol + NAD(P)+
-
 Homo sapiens catechol + NAD(P)H
-
 ?
1.3.1.20 trans-naphthalene dihydrodiol + NAD(P)+
-
 Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.20 monomer 1* 35000, recombinant DD4, 1 * 36000, native DD4, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.3.1.20 DD
-
 Homo sapiens
1.3.1.20 dihydrodiol dehydrogenase
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.20 NADP+
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.3.1.20 0.00008
-
 Phenolphthalein recombinant DD4 Homo sapiens
1.3.1.20 0.15
-
 1-Indanone recombinant DD4 Homo sapiens