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Literature summary extracted from

  • Bresters, T.W.; de Abreu, R.A.; de Kok, A.; Visser, J.; Veeger, C.
    The pyruvate-dehydrogenase complex from Azotobacter vinelandii (1975), Eur. J. Biochem., 59, 335-345.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.104 Mg2+
-
Sus scrofa
1.2.1.104 Mg2+
-
Azotobacter vinelandii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.104 pyruvate + lipoamide Sus scrofa
-
S-acetyldihydrolipoamide + CO2
-
?
1.2.1.104 pyruvate + lipoamide Escherichia coli additional reactions of complex, e. g. reduction of K3Fe(CN)6 S-acetyldihydrolipoamide + CO2
-
?
1.2.1.104 pyruvate + lipoamide Azotobacter vinelandii additional reactions of complex, e. g. reduction of K3Fe(CN)6 S-acetyldihydrolipoamide + CO2
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.104 Azotobacter vinelandii
-
-
-
1.2.1.104 Escherichia coli
-
-
-
1.2.1.104 Sus scrofa
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.104 0.085
-
pyruvate-K3Fe(CN)6 reductase assay Sus scrofa
1.2.1.104 0.14
-
pyruvate-K3Fe(CN)6 reductase assay Sus scrofa
1.2.1.104 0.2
-
pyruvate-K3Fe(CN)6 reductase assay Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.104 pyruvate + lipoamide
-
Sus scrofa S-acetyldihydrolipoamide + CO2
-
?
1.2.1.104 pyruvate + lipoamide additional reactions of complex, e. g. reduction of K3Fe(CN)6 Escherichia coli S-acetyldihydrolipoamide + CO2
-
?
1.2.1.104 pyruvate + lipoamide additional reactions of complex, e. g. reduction of K3Fe(CN)6 Azotobacter vinelandii S-acetyldihydrolipoamide + CO2
-
?

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.104 thiamine diphosphate
-
Escherichia coli
1.2.1.104 thiamine diphosphate
-
Sus scrofa
1.2.1.104 thiamine diphosphate
-
Azotobacter vinelandii