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Literature summary extracted from

  • Ishii, K.; Shiio, I.
    Regulation of purine ribonucleotide synthesis by end product inhibition. I. Effect of purine nucleotides on inosine-5-phosphate dehydrogenase, xanthosine-5-phosphate aminase and adenylosuccinate lyase of Bacillus subtilis (1968), J. Biochem., 63, 661-669.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.205 glutathione removal of glutathione from the reaction mixture causes 35% loss of acitivity Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.205 2'-deoxy-ATP 18% inhibition at 0.5 mM Bacillus subtilis
1.1.1.205 2'-deoxy-GDP 12% inhibition at 0.5 mM Bacillus subtilis
1.1.1.205 2'-deoxy-GTP 21% inhibition at 0.5 mM Bacillus subtilis
1.1.1.205 ATP at high concentration of IMP and KCl Bacillus subtilis
1.1.1.205 GMP competitive, 58% inhibition at 0.5 mM Bacillus subtilis
1.1.1.205 additional information
-
 Bacillus subtilis
1.1.1.205 xanthosine 5'-phosphate 39% competitive, inhibition at 0.5 mM Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.205 0.0256
-
 IMP
-
 Bacillus subtilis
1.1.1.205 0.67
-
 NAD+
-
 Bacillus subtilis
1.1.1.205 10
-
 K+
-
 Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.205 K+
-
 Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.205 Bacillus subtilis
-
 M strain
-
1.1.1.205 Bacillus subtilis M
-
 M strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.205 partial purification Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.205 0.25
-
-
 Bacillus subtilis

Storage Stability

EC Number Storage Stability Organism
1.1.1.205 frozen, 2 weeks, no loss of activity Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.205 inosine 5'-phosphate + NAD+ + H2O
-
 Bacillus subtilis xanthosine 5'-phosphate + NADH
-
 ?
1.1.1.205 inosine 5'-phosphate + NAD+ + H2O
-
 Bacillus subtilis M xanthosine 5'-phosphate + NADH
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.205 2
-
 60% loss of activity within 4 days Bacillus subtilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.205 NAD+ NAD+ can not be replaced by NADP+ Bacillus subtilis