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Literature summary extracted from

  • Twerdochlib, A.L.; Pedrosa, F.O.; Funayama, S.; Rigo, L.U.
    L-Rhamnose metabolism in Pichia stipitis and Debaryomyces polymorphus (1994), Can. J. Microbiol., 40, 896-902.
No PubMed abstract available

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.173 L-rhamnose + NAD+ Scheffersomyces stipitis
-
 L-rhamno-1,4-lactone + NADH product unstable, apparent product L-rhamnonate r
1.1.1.173 L-rhamnose + NAD+ Scheffersomyces stipitis NRC 5568
-
 L-rhamno-1,4-lactone + NADH product unstable, apparent product L-rhamnonate r
3.1.1.65 L-rhamnono-1,4-lactone + H2O Scheffersomyces stipitis oxidative pathway of L-rhamnose L-rhamnonate
-
 r
3.1.1.65 L-rhamnono-1,4-lactone + H2O Schwanniomyces polymorphus oxidative pathway of L-rhamnose L-rhamnonate
-
 r
3.1.1.65 L-rhamnono-1,4-lactone + H2O Scheffersomyces stipitis NRC 5568 oxidative pathway of L-rhamnose L-rhamnonate
-
 r
3.1.1.65 L-rhamnono-1,4-lactone + H2O Schwanniomyces polymorphus 1747 oxidative pathway of L-rhamnose L-rhamnonate
-
 r
4.1.2.53 2-dehydro-3-deoxy-L-rhamnonate Scheffersomyces stipitis
-
 pyruvate + (R)-lactaldehyde
-
 ?
4.1.2.53 2-dehydro-3-deoxy-L-rhamnonate Schwanniomyces polymorphus
-
 pyruvate + (R)-lactaldehyde
-
 ?
4.1.2.53 2-dehydro-3-deoxy-L-rhamnonate Schwanniomyces polymorphus 1747
-
 pyruvate + (R)-lactaldehyde
-
 ?
4.2.1.90 L-rhamnonate Scheffersomyces stipitis
-
 2-dehydro-3-deoxy-L-rhamnonate + H2O
-
 ?
4.2.1.90 L-rhamnonate Schwanniomyces polymorphus
-
 2-dehydro-3-deoxy-L-rhamnonate + H2O
-
 ?
4.2.1.90 L-rhamnonate Scheffersomyces stipitis NRC 5568
-
 2-dehydro-3-deoxy-L-rhamnonate + H2O
-
 ?
4.2.1.90 L-rhamnonate Schwanniomyces polymorphus 1747
-
 2-dehydro-3-deoxy-L-rhamnonate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.173 Scheffersomyces stipitis
-
-
-
3.1.1.65 Scheffersomyces stipitis
-
 yeast, NRC 5568 and mutant PR1
-
3.1.1.65 Schwanniomyces polymorphus
-
 1747
-
3.1.1.65 Schwanniomyces polymorphus 1747
-
 1747
-
4.1.2.53 Scheffersomyces stipitis
-
-
-
4.1.2.53 Scheffersomyces stipitis NRC 5568
-
-
-
4.1.2.53 Schwanniomyces polymorphus
-
-
-
4.1.2.53 Schwanniomyces polymorphus 1747
-
-
-
4.2.1.90 Scheffersomyces stipitis
-
 NRC 5568, PR1 mutant
-
4.2.1.90 Schwanniomyces polymorphus
-
-
-
4.2.1.90 Schwanniomyces polymorphus 1747
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.90 NRC 5568, PR1 mutant, partial Scheffersomyces stipitis
4.2.1.90 NRC 5568, PR1 mutant, partial Schwanniomyces polymorphus
4.2.1.90 strain 1747, partial Scheffersomyces stipitis
4.2.1.90 strain 1747, partial Schwanniomyces polymorphus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.2.53 culture condition:L-rhamnose-grown cell
-
 Scheffersomyces stipitis
-
4.1.2.53 culture condition:L-rhamnose-grown cell
-
 Schwanniomyces polymorphus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.65 0.43
-
 L-rhamnono-gamma-lactonase activity Schwanniomyces polymorphus
3.1.1.65 0.5
-
 L-rhamnonate dehydratase activity Scheffersomyces stipitis
3.1.1.65 0.51
-
 L-rhamnonate dehydratase activity Schwanniomyces polymorphus
4.2.1.90 0.0083
-
-
 Scheffersomyces stipitis
4.2.1.90 0.0085
-
-
 Schwanniomyces polymorphus

Storage Stability

EC Number Storage Stability Organism
4.2.1.90 -20°C Scheffersomyces stipitis
4.2.1.90 -20°C Schwanniomyces polymorphus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.173 L-rhamnose + NAD+
-
 Scheffersomyces stipitis L-rhamno-1,4-lactone + NADH product unstable, apparent product L-rhamnonate r
1.1.1.173 L-rhamnose + NAD+
-
 Scheffersomyces stipitis NRC 5568 L-rhamno-1,4-lactone + NADH product unstable, apparent product L-rhamnonate r
3.1.1.65 L-rhamnonate L-rhamnonate dehydratase reaction Scheffersomyces stipitis 2-dehydro-3-deoxy-L-rhamnonate + H2O
-
 r
3.1.1.65 L-rhamnonate L-rhamnonate dehydratase reaction Scheffersomyces stipitis NRC 5568 2-dehydro-3-deoxy-L-rhamnonate + H2O
-
 r
3.1.1.65 L-rhamnono-1,4-lactone + H2O
-
 Scheffersomyces stipitis L-rhamnonate
-
 r
3.1.1.65 L-rhamnono-1,4-lactone + H2O
-
 Schwanniomyces polymorphus L-rhamnonate
-
 r
3.1.1.65 L-rhamnono-1,4-lactone + H2O oxidative pathway of L-rhamnose Scheffersomyces stipitis L-rhamnonate
-
 r
3.1.1.65 L-rhamnono-1,4-lactone + H2O oxidative pathway of L-rhamnose Schwanniomyces polymorphus L-rhamnonate
-
 r
3.1.1.65 L-rhamnono-1,4-lactone + H2O
-
 Scheffersomyces stipitis NRC 5568 L-rhamnonate
-
 r
3.1.1.65 L-rhamnono-1,4-lactone + H2O oxidative pathway of L-rhamnose Scheffersomyces stipitis NRC 5568 L-rhamnonate
-
 r
3.1.1.65 L-rhamnono-1,4-lactone + H2O
-
 Schwanniomyces polymorphus 1747 L-rhamnonate
-
 r
3.1.1.65 L-rhamnono-1,4-lactone + H2O oxidative pathway of L-rhamnose Schwanniomyces polymorphus 1747 L-rhamnonate
-
 r
4.1.2.53 2-dehydro-3-deoxy-L-rhamnonate
-
 Scheffersomyces stipitis pyruvate + (R)-lactaldehyde
-
 ?
4.1.2.53 2-dehydro-3-deoxy-L-rhamnonate
-
 Schwanniomyces polymorphus pyruvate + (R)-lactaldehyde
-
 ?
4.1.2.53 2-dehydro-3-deoxy-L-rhamnonate
-
 Schwanniomyces polymorphus 1747 pyruvate + (R)-lactaldehyde
-
 ?
4.2.1.90 L-rhamnonate
-
 Scheffersomyces stipitis 2-dehydro-3-deoxy-L-rhamnonate + H2O
-
 ?
4.2.1.90 L-rhamnonate
-
 Schwanniomyces polymorphus 2-dehydro-3-deoxy-L-rhamnonate + H2O
-
 ?
4.2.1.90 L-rhamnonate
-
 Scheffersomyces stipitis NRC 5568 2-dehydro-3-deoxy-L-rhamnonate + H2O
-
 ?
4.2.1.90 L-rhamnonate
-
 Schwanniomyces polymorphus 1747 2-dehydro-3-deoxy-L-rhamnonate + H2O
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.90 30
-
 assay at Scheffersomyces stipitis
4.2.1.90 30
-
 assay at Schwanniomyces polymorphus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.90 7
-
 assay at Scheffersomyces stipitis
4.2.1.90 7
-
 assay at Schwanniomyces polymorphus