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Literature summary extracted from

  • Ikegami, F.; Takayama, K.; Tajima, Ch.; Murakoshi, I.
    Purification and properties of beta-cyano-L-alanine synthase from Spinacia oleracea (1988), Phytochemistry, 27, 2011-2016.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.9 0.73
-
 NaCN
-
 Spinacia oleracea
4.4.1.9 2.3
-
 L-cysteine
-
 Spinacia oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.9 30000
-
 2 * 30000, SDS-PAGE Spinacia oleracea
4.4.1.9 60000
-
 gel filtration Spinacia oleracea

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.9 Spinacia oleracea
-
 spinach
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.9
-
 Spinacia oleracea

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.9 212
-
-
 Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.9 L-cysteine + HCN
-
 Spinacia oleracea H2S + L-3-cyanoalanine
-
 ir
4.4.1.9 L-cysteine + methanethiol
-
 Spinacia oleracea S-methyl-L-cysteine + ?
-
 ?
4.4.1.9 L-cysteine + NaCN
-
 Spinacia oleracea L-3-cyanoalanin + Na2S
-
 ?
4.4.1.9 L-cysteine + pyrazole
-
 Spinacia oleracea beta-(pyrazol-1-yl)-L-alanine + ?
-
 ?
4.4.1.9 additional information
-
 Spinacia oleracea ?
-
 ?
4.4.1.9 O-acetylserine + H2S
-
 Spinacia oleracea cysteine + acetic acid
-
 ir
4.4.1.9 O-acetylserine + HCN
-
 Spinacia oleracea beta-cyanoalanine + acetic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
4.4.1.9 dimer 2 * 30000, SDS-PAGE Spinacia oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4.1.9 9 9.5
-
 Spinacia oleracea

Cofactor

EC Number Cofactor Comment Organism Structure
4.4.1.9 pyridoxal 5'-phosphate 1 mol per mol of subunit Spinacia oleracea