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Literature summary extracted from
- Purification and characterization of fumarase from the syntrophic propionate-oxidizing bacterium strain MPOB (1996), Arch. Microbiol., 165, 126-131.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 4.2.1.2 | exposure to air at room temperature causes 50% loss of activity, reactivation with FeSO4 and 2-mercaptoethanol | Bacteria |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.2 | 0.25 | - |
fumarate | - |
Bacteria |  |
| 4.2.1.2 | 2.38 | - |
L-malate | - |
Bacteria | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.2 | 60000 | - |
2 * 60000, SDS-PAGE | Bacteria |
| 4.2.1.2 | 114000 | - |
gel filtration | Bacteria |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.2 | Bacteria | - |
synthrophic propionate-oxidizing bacterium strain MPOB | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.2 | - |
Bacteria |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.2 | 971 | - |
- |
Bacteria |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 4.2.1.2 | 4°C, under N2, stable for at least 1 month | Bacteria |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.2 | (S)-malate | - |
Bacteria | fumarate + H2O | - |
r | |
| 4.2.1.2 | fumarate + H2O | - |
Bacteria | L-malate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.1.2 | dimer | 2 * 60000, SDS-PAGE | Bacteria |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.2 | 53 | 55 | - |
Bacteria |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.2 | 540 | - |
(S)-malate | - |
Bacteria | |
| 4.2.1.2 | 690 | - |
fumarate | - |
Bacteria | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.2 | 8.5 | - |
- |
Bacteria |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.2 | 5.5 | 11.5 | no activity below pH 5.5 and above pH 11.5 | Bacteria |
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Release 2023.1 (January 2023)
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