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Literature summary extracted from

  • Cook, W.R.; Ramaley, R.F.
    Purification and catalytic properties of "thermostable" fumarase from Bacillus stearothermophilus NU-10 and Thermus X-1 (1976), Experientia, 26, 207-222.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.2 additional information no substrate inhibition by high levels of malate Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.2 180000
-
 gel filtration Geobacillus stearothermophilus
4.2.1.2 180000
-
 gel filtration Thermus sp.

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.2 Geobacillus stearothermophilus
-
 NU-10
-
4.2.1.2 Geobacillus stearothermophilus NU-10
-
 NU-10
-
4.2.1.2 Thermus sp.
-
 X-1
-
4.2.1.2 Thermus sp. X-1
-
 X-1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.2
-
 Geobacillus stearothermophilus
4.2.1.2
-
 Thermus sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.2 additional information
-
-
 Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.2 (S)-malate
-
 Geobacillus stearothermophilus fumarate + H2O
-
 ?
4.2.1.2 (S)-malate
-
 Thermus sp. fumarate + H2O
-
 ?
4.2.1.2 (S)-malate
-
 Thermus sp. X-1 fumarate + H2O
-
 ?
4.2.1.2 (S)-malate
-
 Geobacillus stearothermophilus NU-10 fumarate + H2O
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.2 81
-
-
 Geobacillus stearothermophilus
4.2.1.2 83
-
-
 Thermus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.2 8
-
-
 Geobacillus stearothermophilus
4.2.1.2 8
-
-
 Thermus sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.2 7 10 pH 7: about 60% of maximal activity, pH 10: about 30% of maximal activity Geobacillus stearothermophilus
4.2.1.2 7 10 pH 7: about 60% of maximal activity, pH 10: about 30% of maximal activity Thermus sp.