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Literature summary extracted from

  • Green, G.A.; Girardot, R.; Baldacini, O.; Ledig, M.; Monteil, H.
    Characterization of enolase from Clostridium difficile (1993), Curr. Microbiol., 26, 53-56.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.11 F- in presence of phosphate, competitive Clostridioides difficile

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.11 Mg2+ optimal concentration: 1 mM Clostridioides difficile
4.2.1.11 Zn2+ optimal concentration: 1 mM Clostridioides difficile

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.11 50000
-
 x * 50000, SDS-PAGE Clostridioides difficile
4.2.1.11 300000
-
 non-denaturing PAGE Clostridioides difficile
4.2.1.11 338000
-
 gel filtration Clostridioides difficile

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.11 Clostridioides difficile
-
-
-
4.2.1.11 Paeniclostridium sordellii
-
-
-
4.2.1.11 Paraclostridium bifermentans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.11
-
 Clostridioides difficile
4.2.1.11 partial Paeniclostridium sordellii
4.2.1.11 partial Paraclostridium bifermentans

Storage Stability

EC Number Storage Stability Organism
4.2.1.11 -20°C, 10 mM Tris-HCL, pH 7.5, stable Clostridioides difficile

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.11 2-phospho-D-glycerate
-
 Clostridioides difficile phosphoenolpyruvate
-
 ?
4.2.1.11 2-phospho-D-glycerate
-
 Paeniclostridium sordellii phosphoenolpyruvate
-
 ?
4.2.1.11 2-phospho-D-glycerate
-
 Paraclostridium bifermentans phosphoenolpyruvate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.11 ? x * 50000, SDS-PAGE Clostridioides difficile

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.11 25 50
-
 Clostridioides difficile

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.1.11 25 80 80°C: 27% of maximal activity Clostridioides difficile
4.2.1.11 25 80 25-50°C: maximal activity, 60°C, 89% of maximal activity, 70°C: 57% of maximal activity Clostridioides difficile

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.11 7.6
-
-
 Clostridioides difficile