EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.99.2 | 0.054 | - |
L-Tyr | - |
Symbiobacterium thermophilum | |
4.1.99.2 | 1.67 | - |
S-methyl-L-Cys | - |
Symbiobacterium thermophilum | |
4.1.99.2 | 10.2 | - |
3-chloro-L-Ala | - |
Symbiobacterium thermophilum |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.1.99.2 | K+ | 5-10 mM, accelerates activity | Symbiobacterium thermophilum | |
4.1.99.2 | NH4+ | 5-10 mM, accelerates activity | Symbiobacterium thermophilum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.1.99.2 | 48000 | - |
4 * 48000, SDS-PAGE | Symbiobacterium thermophilum |
4.1.99.2 | 200000 | - |
gel filtration | Symbiobacterium thermophilum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.99.2 | Symbiobacterium thermophilum | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.99.2 | - |
Symbiobacterium thermophilum |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.1.99.2 | 8.133 | - |
- |
Symbiobacterium thermophilum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.99.2 | 3-chloro-L-Ala + H2O | - |
Symbiobacterium thermophilum | ? | - |
? | |
4.1.99.2 | L-Cys + H2O | - |
Symbiobacterium thermophilum | ? + pyruvate + NH3 | - |
? | |
4.1.99.2 | pyrocatechol + pyruvate + NH3 | - |
Symbiobacterium thermophilum | 3,4-dihydroxyphenyl-L-Ala + H2O | - |
? | |
4.1.99.2 | S-methyl-L-Cys + H2O | - |
Symbiobacterium thermophilum | ? + pyruvate + NH3 | - |
? | |
4.1.99.2 | Ser + H2O | L-Ser | Symbiobacterium thermophilum | ? + pyruvate + NH3 | - |
? | |
4.1.99.2 | Tyr + H2O | L-Tyr | Symbiobacterium thermophilum | phenol + pyruvate + NH3 | - |
? | |
4.1.99.2 | Tyr + H2O | D-Tyr | Symbiobacterium thermophilum | phenol + pyruvate + NH3 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.1.99.2 | tetramer | 4 * 48000, SDS-PAGE | Symbiobacterium thermophilum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.99.2 | 80 | - |
- |
Symbiobacterium thermophilum |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.99.2 | 60 | 85 | about 35% of maximal activity at 60°C and 85°C | Symbiobacterium thermophilum |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.99.2 | 80 | - |
20 min, stable up to | Symbiobacterium thermophilum |
4.1.99.2 | 85 | - |
20 min, about 25% loss of activity | Symbiobacterium thermophilum |
4.1.99.2 | 90 | - |
20 min, about 90% loss of activity | Symbiobacterium thermophilum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.1.99.2 | 7 | - |
- |
Symbiobacterium thermophilum |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
4.1.99.2 | 6 | 11 | 25°C, 48 h | Symbiobacterium thermophilum |