EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.1.3.27 | 6-diazo-5-oxo-L-norleucine | - |
Pseudomonas putida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.3.27 | 0.007 | - |
chorismate | enzyme component ASI, with Gln as cosubstrate | Pseudomonas putida | |
4.1.3.27 | 0.09 | - |
chorismate | two-component enzyme, ASI,ASII, with ammonium as cosubstrate | Pseudomonas putida | |
4.1.3.27 | 0.6 | - |
Gln | - |
Pseudomonas putida | |
4.1.3.27 | 26 | - |
NH4+ | two-component enzyme, ASI,ASII | Pseudomonas putida |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.1.3.27 | 18000 | - |
1 * 18000 + 1 * 63400, sucrose density gradient centrifugation | Pseudomonas putida |
4.1.3.27 | 63400 | - |
1 * 18000 + 1 * 63400, sucrose density gradient centrifugation | Pseudomonas putida |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.3.27 | Pseudomonas putida | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.3.27 | - |
Pseudomonas putida |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.1.3.27 | additional information | - |
- |
Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.3.27 | chorismate + L-Gln | - |
Pseudomonas putida | anthranilate + pyruvate + L-glutamate | - |
? | |
4.1.3.27 | chorismate + NH4+ | reaction is catalyzed by component I alone and in combination with component II | Pseudomonas putida | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.1.3.27 | dimer | 1 * 18000 + 1 * 63400, sucrose density gradient centrifugation | Pseudomonas putida |
4.1.3.27 | More | glutamine, 10 mM, enhances binding of AS I and II, as does Mg2+. ASI and ASII play a joint role in the binding of L-Gln, negligible binding to either component alone | Pseudomonas putida |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.1.3.27 | 8.8 | - |
reaction of ASI with chorismate and NH4+ | Pseudomonas putida |