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Literature summary extracted from
- Purification and biochemical characteristics of beta-D-glucosidase from a thermophilic fungus, Thermomyces lanuginosus - SSBP (1999), Biotechnol. Appl. Biochem., 30, 81-87.
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.21 | L-cysteine | 50% increase of activity | Thermomyces lanuginosus |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.21 | beta-D-glucose | competitive inhibition, Ki: 0.55 mM | Thermomyces lanuginosus | |
| 3.2.1.21 | cellobiose | competitive inhibition, Ki: 0.52 mM | Thermomyces lanuginosus | |
| 3.2.1.21 | Hg2+ | complete inhibition at 2 mM | Thermomyces lanuginosus | |
| 3.2.1.21 | N-bromosuccinimide | complete inhibition at 2 mM | Thermomyces lanuginosus | |
| 3.2.1.21 | Salicin | competitive inhibition, Ki: 0.81 mM | Thermomyces lanuginosus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.21 | 0.075 | - |
4-nitrophenyl-beta-D-glucopyranoside | - |
Thermomyces lanuginosus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.21 | extracellular | - |
Thermomyces lanuginosus | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 101000 | - |
2 * 101000, SDS-PAGE | Thermomyces lanuginosus |
| 3.2.1.21 | 200000 | - |
gel filtration | Thermomyces lanuginosus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | 4-O-alpha-D-glucopyranosyl-D-glucose | Thermomyces lanuginosus | maltose | alpha-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | Thermomyces lanuginosus | cellobiose | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 6-O-beta-D-glucopyranosyl-D-glucose + H2O | Thermomyces lanuginosus | low activity | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 6-O-beta-D-glucopyranosyl-D-glucose + H2O | Thermomyces lanuginosus | gentiobiose | beta-D-glucose + D-glucose | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | Thermomyces lanuginosus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.21 | to homogeneity, chromatography steps | Thermomyces lanuginosus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | cell culture | - |
Thermomyces lanuginosus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 9.65 | - |
- |
Thermomyces lanuginosus |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.2.1.21 | 4°C, 20 mM Tris-HCl, pH 7.1, 4 months | Thermomyces lanuginosus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | (2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O | low activity | Thermomyces lanuginosus | 2-hydroxymethylphenol + beta-D-glucose | - |
? | |
| 3.2.1.21 | (2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O | salicin | Thermomyces lanuginosus | 2-hydroxymethylphenol + beta-D-glucose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | best substrate | Thermomyces lanuginosus | 4-nitrophenol + D-glucopyranose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl beta-D-xylopyranoside + H2O | low activity | Thermomyces lanuginosus | 4-nitrophenol + beta-D-xylose | - |
? | |
| 3.2.1.21 | 4-nitrophenyl-alpha-D-glucoside | less, but significant hydrolysis | Thermomyces lanuginosus | 4-nitrophenol + alpha-D-glucose | - |
? | |
| 3.2.1.21 | 4-O-alpha-D-glucopyranosyl-D-glucose | maltose | Thermomyces lanuginosus | alpha-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 4-O-beta-D-glucopyranosyl-D-glucose + H2O | cellobiose | Thermomyces lanuginosus | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 6-O-beta-D-glucopyranosyl-D-glucose + H2O | low activity | Thermomyces lanuginosus | beta-D-glucose + D-glucose | - |
? | |
| 3.2.1.21 | 6-O-beta-D-glucopyranosyl-D-glucose + H2O | gentiobiose | Thermomyces lanuginosus | beta-D-glucose + D-glucose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | dimer | 2 * 101000, SDS-PAGE | Thermomyces lanuginosus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 65 | - |
- |
Thermomyces lanuginosus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 50 | - |
stable within 30 min | Thermomyces lanuginosus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.21 | 44.4 | - |
4-nitrophenyl-beta-D-glucopyranoside | - |
Thermomyces lanuginosus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 6 | - |
- |
Thermomyces lanuginosus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 8 | - |
rapid decrease of activity above | Thermomyces lanuginosus |
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