EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.21 | EDTA | strong activation of both PGI and PGII at 10 mM | Penicillium aurantiogriseum | |
3.2.1.117 | EDTA | activating | Penicillium aurantiogriseum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.21 | Ag+ | strong inhibition at 1 mM for PGI and PGII | Penicillium aurantiogriseum | |
3.2.1.21 | Cu2+ | stronger inhibition for linamarin than for amygdalin at 1 mM, both PGI and PGII | Penicillium aurantiogriseum | |
3.2.1.21 | D-glucono-1,5-lactone | complete inhibition at 1 mM for PGI and PGII | Penicillium aurantiogriseum | |
3.2.1.21 | Fe3+ | strong inhibition at 10 mM for PGI and PGII | Penicillium aurantiogriseum | |
3.2.1.21 | Mn2+ | moderate inhibition at 10 mM for PGI and PGII | Penicillium aurantiogriseum | |
3.2.1.117 | Ag+ | - |
Penicillium aurantiogriseum | |
3.2.1.117 | Co2+ | - |
Penicillium aurantiogriseum | |
3.2.1.117 | Cu2+ | - |
Penicillium aurantiogriseum | |
3.2.1.117 | Fe3+ | - |
Penicillium aurantiogriseum | |
3.2.1.117 | glucono-delta-lactone | complete inhibition at 1 mM | Penicillium aurantiogriseum | |
3.2.1.117 | Mn2+ | - |
Penicillium aurantiogriseum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.21 | 0.43 | - |
amygdalin | for PGI | Penicillium aurantiogriseum | |
3.2.1.21 | 0.47 | - |
amygdalin | for PGII | Penicillium aurantiogriseum | |
3.2.1.21 | 2.32 | - |
linamarin | for PGII | Penicillium aurantiogriseum | |
3.2.1.21 | 2.41 | - |
linamarin | for PGI | Penicillium aurantiogriseum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.2.1.21 | extracellular | most enzyme synthesis if grown in medium containing yeast extract | Penicillium aurantiogriseum | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.21 | Ca2+ | activation for PGI at 10 mM | Penicillium aurantiogriseum | |
3.2.1.21 | Zn2+ | activation at 10 mM for both PGI and PGII | Penicillium aurantiogriseum | |
3.2.1.117 | Ca2+ | activating | Penicillium aurantiogriseum | |
3.2.1.117 | Zn2+ | activating | Penicillium aurantiogriseum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 61100 | - |
4 * 61100, SDS-PAGE, for PGI | Penicillium aurantiogriseum |
3.2.1.21 | 71000 | - |
2 * 71000, SDS-PAGE, for PGII | Penicillium aurantiogriseum |
3.2.1.21 | 147000 | - |
for PGII, gel filtration | Penicillium aurantiogriseum |
3.2.1.21 | 247000 | - |
for PGI, gel filtration | Penicillium aurantiogriseum |
3.2.1.117 | 147000 | - |
PG II, gel filtration | Penicillium aurantiogriseum |
3.2.1.117 | 247000 | - |
PG I, gel filtration | Penicillium aurantiogriseum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.117 | (R)-amygdalin + H2O | Penicillium aurantiogriseum | first step of cyanogenesis from amygdalin | (R)-prunasin + D-glucose | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.21 | Penicillium aurantiogriseum | - |
- |
- |
3.2.1.117 | Penicillium aurantiogriseum | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.21 | to homogeneity, preparative isoelectric focusing, 1step chromatography | Penicillium aurantiogriseum |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.2.1.117 | (R)-amygdalin + H2O = (R)-prunasin + D-glucose | extracellular enzyme that shows amygdalin as well as prunasin hydrolase activity | Penicillium aurantiogriseum |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.21 | cell culture | 2 isoenzymes, PGI and PGII | Penicillium aurantiogriseum | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 0.16 | - |
for PGI | Penicillium aurantiogriseum |
3.2.1.21 | 0.41 | - |
for PGII | Penicillium aurantiogriseum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.21 | (R)-alpha-[(6-O-beta-D-glucopyranosyl-beta-D-glucopyranosyl)oxy]benzeneacetonitrile + H2O | amygdalin | Penicillium aurantiogriseum | HCN + ? | - |
? | |
3.2.1.21 | cardiospermin sulfate + H2O | low activity | Penicillium aurantiogriseum | HCN + ? | - |
? | |
3.2.1.21 | dhurrin + H2O | low activity for PGI | Penicillium aurantiogriseum | HCN + beta-D-glucose + 4-hydroxybenzaldehyde | - |
? | |
3.2.1.21 | epivolkenin + H2O | low activity | Penicillium aurantiogriseum | HCN + ? | - |
? | |
3.2.1.21 | gynocardin + H2O | low activity | Penicillium aurantiogriseum | HCN + ? | - |
? | |
3.2.1.21 | linamarin + H2O | low activity | Penicillium aurantiogriseum | HCN + ? | - |
? | |
3.2.1.21 | lucumin + H2O | low activity for PGI, very low activity for PGII | Penicillium aurantiogriseum | HCN + ? | - |
? | |
3.2.1.21 | passibiflorin + H2O | low activity | Penicillium aurantiogriseum | HCN + ? | - |
? | |
3.2.1.21 | prunasin + H2O | - |
Penicillium aurantiogriseum | D-mandelonitrile + beta-D-glucose | - |
? | |
3.2.1.21 | samibunigrin + H2O | best substrate for PGI and PGII | Penicillium aurantiogriseum | HCN + ? | - |
? | |
3.2.1.21 | taxiphyllin + H2O | low activity for PGI | Penicillium aurantiogriseum | HCN + ? | - |
? | |
3.2.1.21 | tetraphyllin + H2O | - |
Penicillium aurantiogriseum | HCN + ? | - |
? | |
3.2.1.117 | (R)-amygdalin + H2O | very broad specificity | Penicillium aurantiogriseum | (R)-prunasin + D-glucose | - |
? | |
3.2.1.117 | (R)-amygdalin + H2O | first step of cyanogenesis from amygdalin | Penicillium aurantiogriseum | (R)-prunasin + D-glucose | - |
? | |
3.2.1.117 | (R)-prunasin + H2O | very effective substrate, EC 3.2.1.118 activity | Penicillium aurantiogriseum | D-glucose + mandelonitrile | - |
? | |
3.2.1.117 | cardiospermin 5-sulfate + H2O | poor substrate | Penicillium aurantiogriseum | D-glucose + sulfuric acid mono-(3-cyano-3-hydroxy-2-methylenepropyl) ester | - |
? | |
3.2.1.117 | dhurrin + H2O | - |
Penicillium aurantiogriseum | D-glucose + 4-hydroxymandelonitrile | - |
? | |
3.2.1.117 | epivolkenin + H2O | poor substrate | Penicillium aurantiogriseum | D-glucose + 1,4-dihydroxycyclopent-2-enecabonitrile | - |
? | |
3.2.1.117 | gynocardin + H2O | - |
Penicillium aurantiogriseum | D-glucose + 1,4,5-hydroxycyclopent-2-enecarbonitrile | - |
? | |
3.2.1.117 | linamarin + H2O | poor substrate | Penicillium aurantiogriseum | D-glucose + acetone cyanohydrin | - |
? | |
3.2.1.117 | lucumin + H2O | poor substrate | Penicillium aurantiogriseum | ? | - |
? | |
3.2.1.117 | passibiflorin + H2O | poor substrate | Penicillium aurantiogriseum | D-glucose + 1,4-dihydroxycyclopent-2-enecabonitrile | - |
? | |
3.2.1.117 | sambunigrin + H2O | very effective substrate | Penicillium aurantiogriseum | D-glucose + mandelonitrile | - |
? | |
3.2.1.117 | taxiphyllin + H2O | - |
Penicillium aurantiogriseum | D-glucose + 4-hydroxymandelonitrile | - |
? | |
3.2.1.117 | tetraphyllin B + H2O | very effective substrate | Penicillium aurantiogriseum | D-glucose + 1,4-dihydroxycyclopent-2-enecabonitrile | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.21 | dimer | 2 * 71000, SDS-PAGE, for PGII | Penicillium aurantiogriseum |
3.2.1.21 | tetramer | 4 * 61100, SDS-PAGE, for PGI | Penicillium aurantiogriseum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.21 | linamarase | - |
Penicillium aurantiogriseum |
3.2.1.21 | PGI | - |
Penicillium aurantiogriseum |
3.2.1.21 | PGII | - |
Penicillium aurantiogriseum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 55 | - |
for PGI | Penicillium aurantiogriseum |
3.2.1.21 | 60 | - |
for PGII | Penicillium aurantiogriseum |
3.2.1.117 | 55 | - |
PG I | Penicillium aurantiogriseum |
3.2.1.117 | 60 | - |
PG II | Penicillium aurantiogriseum |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 50 | - |
for PGI, stable up to | Penicillium aurantiogriseum |
3.2.1.21 | 60 | - |
for PGII, stable up to | Penicillium aurantiogriseum |
3.2.1.117 | 55 | - |
PG I, half life: 2 h | Penicillium aurantiogriseum |
3.2.1.117 | 60 | - |
PG II, half life: 7 h | Penicillium aurantiogriseum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 6 | - |
for both PGI and PGII | Penicillium aurantiogriseum |
3.2.1.117 | 6 | - |
both isoforms | Penicillium aurantiogriseum |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 5 | 7.5 | for PGI | Penicillium aurantiogriseum |
3.2.1.21 | 5.5 | 7.5 | for PGII | Penicillium aurantiogriseum |