Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Grifantini, R.; Pratesi, C.; Galli, G.; Grandi, G.
    Topological mapping of the cysteine residues of N-carbamoyl-D-amino-acid amidohydrolase and their role in enzymatic activity (1996), J. Biol. Chem., 271, 9326-9331.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.1.77 synthesis enzyme is used for the industrial production of D-amino acids Agrobacterium tumefaciens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.77 expression in Escherichia coli Agrobacterium tumefaciens

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.77 C172A mutant enzyme Cys172Ala or Cys172Ser is completely inactive. Substitution of any of the other Cys has no effect on enzyme activity Agrobacterium tumefaciens
3.5.1.77 C172S mutant enzyme Cys172Ala or Cys172Ser is completely inactive. Substitution of any of the other Cys has no effect on enzyme activity Agrobacterium tumefaciens

General Stability

EC Number General Stability Organism
3.5.1.77 stable in absence rather than in presence of 2-mercaptoethanol Agrobacterium tumefaciens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.77 iodoacetate
-
 Agrobacterium tumefaciens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.77 32000
-
 2 * 32000, SDS-PAGE Agrobacterium tumefaciens
3.5.1.77 34000
-
 2 * 34000, calculation from nucleotide sequence Agrobacterium tumefaciens
3.5.1.77 67000
-
 gel filtration Agrobacterium tumefaciens

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.77 Agrobacterium tumefaciens
-
-
-
3.5.1.77 Agrobacterium tumefaciens NTRRL B11291
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.77 10.1
-
-
 Agrobacterium tumefaciens

Storage Stability

EC Number Storage Stability Organism
3.5.1.77 4°C, phosphate buffer, progressive loss of activity Agrobacterium tumefaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.77 N-carbamoyl-D-p-hydroxyphenylglycine + H2O
-
 Agrobacterium tumefaciens D-p-hydroxyphenylglycine + NH3 + CO2
-
 ?
3.5.1.77 N-carbamoyl-D-p-hydroxyphenylglycine + H2O
-
 Agrobacterium tumefaciens NTRRL B11291 D-p-hydroxyphenylglycine + NH3 + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.77 dimer 2 * 32000, SDS-PAGE Agrobacterium tumefaciens
3.5.1.77 dimer 2 * 34000, calculation from nucleotide sequence Agrobacterium tumefaciens