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Literature summary extracted from

  • Willis, T.W.; Tu, A.T.
    Purification and biochemical characterization of atroxase, a nonhemorrhagic fibrinolytic protease from western diamondback rattlesnake venom (1988), Biochemistry, 27, 4769-4777.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.43 alpha2-Macroglobulin
-
 Crotalus atrox
3.4.24.43 EDTA Zn2+ protects Crotalus atrox
3.4.24.43 additional information no inhibition by aprotinin, PMSF or soybean trypsin inhibitor Crotalus atrox

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.43 Ca2+ 0.3 mol/mol enzyme Crotalus atrox
3.4.24.43 K+ 1 mol/mol enzyme Crotalus atrox
3.4.24.43 Zn2+ class I zinc metalloproteinase, contains 1 mol zinc/mol enzyme, no activation by added Zn2+ Crotalus atrox

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.43 additional information
-
 amino acid composition Crotalus atrox
3.4.24.43 23500
-
 x * 23500, Crotalus atrox, SDS-PAGE Crotalus atrox

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.43 additional information Crotalus atrox nontoxic venom component, nonhemorrhagic endopeptidase ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.43 Crotalus atrox
-
 Western diamond rattlesnake
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.43 from lyophilized crude venom Crotalus atrox

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.43 venom
-
 Crotalus atrox
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.43 additional information
-
 1.67 mg fibrinogen/min mg enzyme Crotalus atrox

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.43 Dimethylcasein + H2O
-
 Crotalus atrox ?
-
 ?
3.4.24.43 Human fibrinogen + H2O gamma chain is not hydrolyzed Crotalus atrox ?
-
 ?
3.4.24.43 Human fibrinogen + H2O hydrolysis of Aalpha and Bbeta chain, cleavage pattern different from thrombin cleavage pattern Crotalus atrox ?
-
 ?
3.4.24.43 additional information no hemorrhagic activity, no substrates are thrombin, synthetic substrate for thrombin S-2238 Crotalus atrox ?
-
 ?
3.4.24.43 additional information nontoxic venom component, nonhemorrhagic endopeptidase Crotalus atrox ?
-
 ?
3.4.24.43 Oxidized insulin B-chain cleavage of His5-Leu6, Ser9-His10, His10-Leu11, Ala14-Leu15, Tyr16-Leu17 Crotalus atrox ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.43 ? x * 23500, Crotalus atrox, SDS-PAGE Crotalus atrox

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.43 37
-
 assay at Crotalus atrox
3.4.24.43 55
-
-
 Crotalus atrox

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.24.43 70
-
 inactivation Crotalus atrox

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.43 additional information
-
 pI: 9.6 Crotalus atrox
3.4.24.43 9
-
-
 Crotalus atrox

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.24.43 11
-
 inactivation Crotalus atrox