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Literature summary extracted from

  • Oda, K.; Torishima, H.; Murao, S.
    Purification and characterization of acid proteinase C of Scytalidium lignicolum ATCC 24568 (1986), Agric. Biol. Chem., 50, 651-658.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.23.31 alpha-Microbial alkaline proteinase inhibitor weak Scytalidium lignicola
3.4.23.31 additional information a related enzyme from the same organism, proteinase C is also insensitive to these inhibitors; diazoacetyl-DL-norleucine methyl ester; methyl 2-diazoacetamidohexanoate, 1,2-epoxy-3-(4-nitrophenyl)propane; Streptomyces-pepsin inhibitor; talopeptin (MK-1, metal proteinase inhibitor); thiolstatin (thiol proteinase inhibitor) Scytalidium lignicola

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.23.31 0.5
-
 Succinyl-Phe-Arg-Ala-Phe 4-nitrophenyl ester enzyme form C Scytalidium lignicola
3.4.23.31 1
-
 Succinyl-Phe-Arg-Ala-Phe 4-nitrophenyl ester enzyme form A-2 Scytalidium lignicola

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.23.31 36000
-
 Scytalidium lignicolum, gel filtration Scytalidium lignicola
3.4.23.31 40600
-
 Scytalidium lignicolum, sedimentation equilibrium analysis Scytalidium lignicola

Organism

EC Number Organism UniProt Comment Textmining
3.4.23.31 Scytalidium lignicola
-
 A-2
-
3.4.23.31 Scytalidium lignicola
-
 C
-
3.4.23.31 Scytalidium lignicola
-
 ATCC 24568
-
3.4.23.31 Scytalidium lignicola A-2
-
 A-2
-
3.4.23.31 Scytalidium lignicola C
-
 C
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.23.31 glycoprotein 30% (expressed as the glucose equivalent) Scytalidium lignicola

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.23.31 proteinase C Scytalidium lignicola

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.23.31 culture filtrate
-
 Scytalidium lignicola
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.23.31 additional information
-
-
 Scytalidium lignicola

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.23.31 N-tert-Butoxycarbonyl-Phe-Arg(NO2)-Ala-Phe 4-nitrophenyl ester + H2O
-
 Scytalidium lignicola ?
-
 ?
3.4.23.31 N-tert-Butoxycarbonyl-Phe-Arg(NO2)-Ala-Phe 4-nitrophenyl ester + H2O
-
 Scytalidium lignicola C ?
-
 ?
3.4.23.31 N-tert-Butoxycarbonyl-Phe-Arg(NO2)-Ala-Phe 4-nitrophenyl ester + H2O
-
 Scytalidium lignicola A-2 ?
-
 ?
3.4.23.31 N-tert-Butoxycarbonyl-Phe-Arg(NO2)-Gly-Phe 4-nitrophenyl ester + H2O
-
 Scytalidium lignicola ?
-
 ?
3.4.23.31 N-tert-Butoxycarbonyl-Phe-Arg(NO2)-Gly-Phe 4-nitrophenyl ester + H2O
-
 Scytalidium lignicola C ?
-
 ?
3.4.23.31 N-tert-Butoxycarbonyl-Phe-Arg(NO2)-Gly-Phe 4-nitrophenyl ester + H2O
-
 Scytalidium lignicola A-2 ?
-
 ?
3.4.23.31 Succinyl-Phe-Arg-Ala-Phe 4-nitrophenyl ester + H2O at high rate Scytalidium lignicola ?
-
 ?
3.4.23.31 Succinyl-Phe-Arg-Ala-Phe 4-nitrophenyl ester + H2O at high rate Scytalidium lignicola C ?
-
 ?
3.4.23.31 Succinyl-Phe-Arg-Ala-Phe 4-nitrophenyl ester + H2O at high rate Scytalidium lignicola A-2 ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.23.31 50
-
-
 Scytalidium lignicola

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.23.31 37
-
 pH 3-5, 20 h, stable Scytalidium lignicola
3.4.23.31 55
-
 pH 4.8, 15 min, stable below Scytalidium lignicola
3.4.23.31 68
-
 pH 4.8, 15 min, complete loss of activity Scytalidium lignicola

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.23.31 2
-
 casein Scytalidium lignicola

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.23.31 3 5 37°C, 20 h, stable Scytalidium lignicola
3.4.23.31 4.8
-
 15 min, stable below 55°C, complete loss of activity at 68°C Scytalidium lignicola