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Literature summary extracted from

  • Ottesen, M.; Rickert, W.
    Acid protease of Mucor miehei (1970), Methods Enzymol., 19, 459-460.
No PubMed abstract available

General Stability

EC Number General Stability Organism
3.4.23.23 Urea, pH 6.0, 11 h stable Rhizomucor miehei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.23.23 additional information
-
-
 Rhizomucor miehei

Organism

EC Number Organism UniProt Comment Textmining
3.4.23.23 Rhizomucor miehei
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.23.23 glycoprotein 6% covalently bound carbohydrate (hexosamine and neutral hexoses) Rhizomucor miehei

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.23.23 commercial preparation
-
 Rhizomucor miehei
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.23.23 additional information specificity against aromatic bulky or hydrophobic amino acid residues at both sides of the splitting point Rhizomucor miehei ?
-
 ?
3.4.23.23 Oxidized insulin B-chain + H2O hydrolysis at: Phe1-Val2, Ala14-Leu15, Leu15-Tyr16, Tyr16-Leu17, Phe24-Phe25, Phe25-Tyr26, Tyr26-Thr27 Rhizomucor miehei Hydrolyzed oxidized B-chain of insulin
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.23.23 4
-
 hemoglobin Rhizomucor miehei
3.4.23.23 4
-
 insulin B-chain Rhizomucor miehei

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.23.23 3 6 38°C, 8 days, more than 90% of the activity retained Rhizomucor miehei