Any feedback?
Literature summary extracted from
- Purification and characterization of the two molecular forms of Aspergillus oryzae acid protease (1976), Biochim. Biophys. Acta, 445, 194-204.
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.4.23.18 | Aspergillus oryzae | Aspergillus oryzae |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.23.18 | additional information | diisopropyl phosphofluoridate; EDTA; HgCl2, NaF, FeCl3, FeCl2, CoCl2, MgCl2, CaCl2, ZnSO4; monoiodoacetate, 1,10-phenanthroline, 6-aminohexanoate; PCMB | Aspergillus oryzae | |
| 3.4.23.18 | N-bromosuccinimide | not | Aspergillus oryzae |  |
| 3.4.23.18 | SDS | - |
Aspergillus oryzae | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.23.18 | additional information | no activation by HgCl2, NaF, FeCl3, FeCl2, CoCl2, MgCl2, CaCl2, ZnSO4 | Aspergillus oryzae |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.18 | additional information | - |
amino acid composition | Aspergillus oryzae |
| 3.4.23.18 | 32000 | - |
Aspergillus oryzae, enzyme form A2, sedimentation analysis | Aspergillus oryzae |
| 3.4.23.18 | 63000 | - |
Aspergillus oryzae, enzyme form A1, sedimetation analysis | Aspergillus oryzae |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.23.18 | Aspergillus oryzae | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.4.23.18 | glycoprotein | carbohydrate content: less than 0.3% (enzyme form A2), about 50% (enzyme form A1) | Aspergillus oryzae |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.23.18 | several isoforms (extracellular A1 and A2) | Aspergillus oryzae |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.23.18 | commercial preparation | Taka-diastase, crude Aspergillus oryzae powder preparation | Aspergillus oryzae | - |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.4.23.18 | -20°C, in 50 mM sodium acetate, pH 5, stable | Aspergillus oryzae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.23.18 | casein + H2O | - |
Aspergillus oryzae | ? | - |
? | |
| 3.4.23.18 | Cytochrome c + H2O | from horse heart | Aspergillus oryzae | ? | - |
? | |
| 3.4.23.18 | Hemoglobin + H2O | urea-denatured hemoglobin | Aspergillus oryzae | ? | - |
? | |
| 3.4.23.18 | Trypsinogen + H2O | bovine (pancreatic) | Aspergillus oryzae | ? | - |
? | |
| 3.4.23.18 | Trypsinogen + H2O | bovine (trypsinogen) | Aspergillus oryzae | ? | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.18 | 55 | - |
10 min, in 50 mM sodium acetate buffer, pH 5 | Aspergillus oryzae |
| 3.4.23.18 | 55 | - |
isozyme A1 loses 10% of its original activity in contrast to almost complete loss of activity of isozyme A2 | Aspergillus oryzae |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.18 | additional information | - |
pI: 3.15 and 3.5 (isozyme A1) | Aspergillus oryzae |
| 3.4.23.18 | additional information | - |
pI: 3.9 (isozyme A2) | Aspergillus oryzae |
| 3.4.23.18 | 3 | - |
cytochrome c | Aspergillus oryzae |
| 3.4.23.18 | 4.2 | - |
urea-denatured hemoglobin | Aspergillus oryzae |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
