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Literature summary extracted from

  • Morihara, K.; Oka, T.
    Comparative specificity of microbial acid proteinases for synthetic peptides. 3. Relationship with their trypsinogen activating ability (1973), Arch. Biochem. Biophys., 157, 561-572.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.23.18 3.4
-
 Benzyloxycarbonyl-Ala-Lys-Ala-Ala-Ala pH 3.2, Aspergillus niger Aspergillus niger
3.4.23.18 3.5
-
 Benzyloxycarbonyl-Ala-Ala-Lys-Ala-Ala-Ala pH 3.2, Aspergillus niger Aspergillus niger
3.4.23.18 4.1 4.3 Benzyloxycarbonyl-Lys-Leu-Ala-Ala Aspergillus niger Aspergillus niger
3.4.23.18 4.5
-
 Benzyloxycarbonyl-Ala-Ala-Lys-Ala-Ala-Ala pH 4.5, Aspergillus niger Aspergillus niger
3.4.23.18 8.2
-
 Benzyloxycarbonyl-Lys-Ala-Ala-Ala pH 3.2, Aspergillus niger Aspergillus niger
3.4.23.18 10.6
-
 Benzyloxycarbonyl-Lys-Ala-Ala-Ala pH 4.5, Aspergillus niger Aspergillus niger
3.4.23.18 10.9
-
 Benzyloxycarbonyl-Ala-Lys-Ala-Ala-Ala pH 4.5, Aspergillus niger Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.4.23.18 Aspergillus niger
-
-
-
3.4.23.18 Aspergillus niger B
-
-
-
3.4.23.18 Aspergillus phoenicis
-
-
-
3.4.23.23 Rhizomucor miehei
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.23.18 Benzyloxycarbonyl-Ala-Ala-Lys-Ala-Ala-Ala + H2O best substrate Aspergillus niger Benzyloxycarbonyl-Ala-Ala-Lys + Ala-Ala-Ala
-
 ?
3.4.23.18 Benzyloxycarbonyl-Ala-Lys-Ala-Ala-Ala + H2O
-
 Aspergillus niger Benzyloxycarbonyl-Ala-Lys + Ala-Ala-Ala
-
 ?
3.4.23.18 Benzyloxycarbonyl-Lys-Ala-Ala + H2O poor substrate Aspergillus niger Benzyloxycarbonyl-Lys + Ala-Ala
-
 ?
3.4.23.18 Benzyloxycarbonyl-Lys-Ala-Ala + H2O poor substrate Aspergillus phoenicis Benzyloxycarbonyl-Lys + Ala-Ala
-
 ?
3.4.23.18 Benzyloxycarbonyl-Lys-Ala-Ala-Ala + H2O
-
 Aspergillus niger Benzyloxycarbonyl-Lys + Ala-Ala-Ala
-
 ?
3.4.23.18 Benzyloxycarbonyl-Lys-Ala-Ala-Ala-Ala + H2O poor substrate Aspergillus niger Benzyloxycarbonyl-Lys + Ala-Ala-Ala-Ala
-
 ?
3.4.23.18 Benzyloxycarbonyl-Lys-Leu-Ala + H2O
-
 Aspergillus niger Benzyloxycarbonyl-Lys + Leu-Ala
-
 ?
3.4.23.18 Benzyloxycarbonyl-Lys-Leu-Ala + H2O
-
 Aspergillus phoenicis Benzyloxycarbonyl-Lys + Leu-Ala
-
 ?
3.4.23.18 Benzyloxycarbonyl-Lys-Leu-Ala + H2O
-
 Aspergillus niger B Benzyloxycarbonyl-Lys + Leu-Ala
-
 ?
3.4.23.18 Benzyloxycarbonyl-Lys-Leu-Ala-Ala + H2O
-
 Aspergillus niger Benzyloxycarbonyl-Lys + Leu-Ala-Ala
-
 ?
3.4.23.18 Benzyloxycarbonyl-Lys-Phe-Ala + H2O
-
 Aspergillus niger Benzyloxycarbonyl-Lys + Phe-Ala
-
 ?
3.4.23.18 Benzyloxycarbonyl-Lys-Phe-Ala + H2O
-
 Aspergillus phoenicis Benzyloxycarbonyl-Lys + Phe-Ala
-
 ?
3.4.23.18 Benzyloxycarbonyl-Lys-Phe-Ala + H2O
-
 Aspergillus niger B Benzyloxycarbonyl-Lys + Phe-Ala
-
 ?
3.4.23.18 additional information specificity Aspergillus niger ?
-
 ?
3.4.23.18 additional information specificity Aspergillus phoenicis ?
-
 ?
3.4.23.18 additional information no hydrolysis of benzyloxycarbonyl-Lys-Gly-Ala, benzyloxycarbonyl-Lys-(D)-Leu-Ala Aspergillus niger ?
-
 ?
3.4.23.18 additional information no hydrolysis of benzyloxycarbonyl-Lys-Gly-Ala, benzyloxycarbonyl-Lys-(D)-Leu-Ala Aspergillus phoenicis ?
-
 ?
3.4.23.18 additional information specificity Aspergillus niger B ?
-
 ?
3.4.23.18 additional information no hydrolysis of benzyloxycarbonyl-Lys-Gly-Ala, benzyloxycarbonyl-Lys-(D)-Leu-Ala Aspergillus niger B ?
-
 ?
3.4.23.18 Trypsinogen + H2O
-
 Aspergillus niger ?
-
 ?
3.4.23.18 Trypsinogen + H2O
-
 Aspergillus phoenicis ?
-
 ?
3.4.23.18 Trypsinogen + H2O
-
 Aspergillus niger B ?
-
 ?
3.4.23.23 additional information specificity against aromatic bulky or hydrophobic amino acid residues at both sides of the splitting point Rhizomucor miehei ?
-
 ?
3.4.23.23 additional information no activation of trypsinogen Rhizomucor miehei ?
-
 ?
3.4.23.23 Peptides + H2O
-
 Rhizomucor miehei Hydrolyzed peptides
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.23.18 40
-
 assay at Aspergillus niger
3.4.23.18 40
-
 assay at Aspergillus phoenicis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.23.18 0.044
-
 Benzyloxycarbonyl-Lys-Ala-Ala-Ala pH 3.2, Aspergillus niger Aspergillus niger
3.4.23.18 0.17
-
 Benzyloxycarbonyl-Lys-Ala-Ala-Ala pH 4.5, Aspergillus niger Aspergillus niger
3.4.23.18 0.38
-
 Benzyloxycarbonyl-Lys-Leu-Ala-Ala pH 3.2, Aspergillus niger Aspergillus niger
3.4.23.18 0.46
-
 Benzyloxycarbonyl-Ala-Lys-Ala-Ala-Ala pH 3.2, Aspergillus niger Aspergillus niger
3.4.23.18 0.67
-
 Benzyloxycarbonyl-Lys-Leu-Ala-Ala pH 4.5, Aspergillus niger Aspergillus niger
3.4.23.18 2.18
-
 Benzyloxycarbonyl-Ala-Lys-Ala-Ala-Ala pH 4.5, Aspergillus niger Aspergillus niger
3.4.23.18 9.32
-
 Benzyloxycarbonyl-Ala-Ala-Lys-Ala-Ala-Ala pH 3.2, Aspergillus niger Aspergillus niger
3.4.23.18 30.5
-
 Benzyloxycarbonyl-Ala-Ala-Lys-Ala-Ala-Ala pH 4.5, Aspergillus niger Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.23.18 4 5 synthetic peptides Aspergillus niger