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Literature summary extracted from

  • Ornston, L.N.
    The conversion of catechol and protocatechuate to beta-ketoadipate by Pseudomonas putida (1966), J. Biol. Chem., 241, 3795-3799.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.3.3.4
-
 Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.3.4 93000
-
 gel filtration Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
5.3.3.4 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.3.4
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.3.4 707
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.3.4 muconolactone
-
 Pseudomonas putida (5-oxo-4,5-dihydrofuran-2-yl)acetic acid
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.3.3.4 55
-
 half-life: 2.5 h Pseudomonas putida
5.3.3.4 65
-
 rapid inactivation at Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.3.4 7.5 8.5
-
 Pseudomonas putida

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.3.3.4 7 9.5 pH 7: about 80% of maximal activity, pH 9.5: about 70% of maximal activity Pseudomonas putida