EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.4.21.53 | Adenosine 2',3'-dialdehyde triphosphate | activation, hydrolysis at 23% the rate of ATP, supports proteolysis with 30% the efficiency of ATP | Escherichia coli | |
3.4.21.53 | GTP | activation | Escherichia coli | |
3.4.21.53 | GTP | hydrolysis at 113% the rate of ATP, supports proteolysis with 14% the efficiency of ATP | Escherichia coli | |
3.4.21.53 | Protein substrates | activation | Escherichia coli | |
3.4.21.53 | Protein substrates | stimulation of ATP hydrolysis triggers activation of the proteolytic function | Escherichia coli | |
3.4.21.53 | spermidine | activation, at physiological concentration, ATP hydrolysis and protease activity | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.21.53 | adenosine 5'-[beta,gamma-imido]triphosphate | protein and ATP hydrolysis | Escherichia coli | |
3.4.21.53 | ADP | protein and ATP hydrolysis | Escherichia coli | |
3.4.21.53 | beta,gamma-methylene-ATP | not | Escherichia coli | |
3.4.21.53 | Dansyl fluoride | protein and ATP hydrolysis | Escherichia coli | |
3.4.21.53 | diisopropyl fluorophosphate | protein and ATP hydrolysis | Escherichia coli | |
3.4.21.53 | iodoacetamide | at high concentration, protein and ATP-hydrolysis | Escherichia coli | |
3.4.21.53 | NaCl | at high concentrations | Escherichia coli | |
3.4.21.53 | vanadate | casein and ATP hydrolysis | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.21.53 | 0.027 | - |
ATP | ATP hydrolysis | Escherichia coli | |
3.4.21.53 | 0.045 | - |
ATP | protein hydrolysis | Escherichia coli |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.21.53 | Mg2+ | requirement | Escherichia coli | |
3.4.21.53 | Mg2+ | 5-7 mM | Escherichia coli | |
3.4.21.53 | Mg2+ | ATP hydrolysis and protease activity | Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.21.53 | 94000 | - |
x * 94000, SDS-PAGE | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.21.53 | additional information | Escherichia coli | ATP-dependent serine protease | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.21.53 | Escherichia coli | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.21.53 | - |
Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.21.53 | ATP + H2O | - |
Escherichia coli | phosphate + ADP | - |
? | |
3.4.21.53 | casein + H2O | alpha-casein | Escherichia coli | hydrolyzed casein | - |
? | |
3.4.21.53 | Denatured albumin + H2O | - |
Escherichia coli | ? | - |
? | |
3.4.21.53 | Denatured bovine serum albumin + H2O | - |
Escherichia coli | ? | - |
? | |
3.4.21.53 | Globin + H2O | - |
Escherichia coli | ? | - |
? | |
3.4.21.53 | Glucagon + H2O | - |
Escherichia coli | Hydrolyzed glucagon | - |
? | |
3.4.21.53 | additional information | ATP-dependent serine protease | Escherichia coli | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.21.53 | multimer | x * 94000, SDS-PAGE | Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.21.53 | 30 | - |
assay at | Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
3.4.21.53 | ATP | requirement | Escherichia coli | |
3.4.21.53 | ATP | activation only in the presence of Mg2+ | Escherichia coli | |
3.4.21.53 | ATP | ATP-dependent protease | Escherichia coli | |
3.4.21.53 | ATP | ATP hydrolysis is essential for hydrolysis of proteins | Escherichia coli | |
3.4.21.53 | CTP | activation | Escherichia coli | |
3.4.21.53 | CTP | hydrolysis at 82% the rate of ATP, supports proteolysis with 31% the efficiency of ATP | Escherichia coli | |
3.4.21.53 | CTP | less efficient than ATP | Escherichia coli | |
3.4.21.53 | dATP | activation | Escherichia coli | |
3.4.21.53 | dATP | supports proteolysis with 92% the efficiency of ATP | Escherichia coli | |
3.4.21.53 | UTP | activation | Escherichia coli | |
3.4.21.53 | UTP | less efficient than ATP | Escherichia coli | |
3.4.21.53 | UTP | hydrolysis at 77% the rate of ATP, supports proteolysis with 22% the efficiency of ATP | Escherichia coli |