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Literature summary extracted from

  • Gilead, S.; Shoham, Y.
    Purification and characterization of alpha-L-arabinofuranosidase from Bacillus stearothermophilus T-6 (1995), Appl. Environ. Microbiol., 61, 170-174.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.55 L-arabinose inducer of AF activity Butyrivibrio fibrisolvens
3.2.1.55 L-arabinose inducer of AF activity Trichoderma reesei
3.2.1.55 xylan induces enzyme activity Butyrivibrio fibrisolvens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.55
-
 Streptomyces lividans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.55 Fe2+ 1 mM FeSO4 inhibits the activity by 25% Geobacillus stearothermophilus
3.2.1.55 Hg2+ 1 mM HgCl2 inhibits the activity by 98% Geobacillus stearothermophilus
3.2.1.55 Hg2+
-
 Streptomyces purpurascens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.55 0.42
-
 p-nitrophenyl-alpha-L-arabinofuranoside
-
 Geobacillus stearothermophilus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.55 cytoplasm
-
 Geobacillus stearothermophilus 5737
-
3.2.1.55 cytoplasm
-
 Butyrivibrio fibrisolvens 5737
-
3.2.1.55 cytoplasm
-
 Streptomyces lividans 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.55 Ca2+ weak stimulation of the enzyme activity Geobacillus stearothermophilus
3.2.1.55 Mg2+ small stimulating effect Geobacillus stearothermophilus
3.2.1.55 Mn2+ small stimulating effect Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.55 64000
-
 4 * 64000, SDS-PAGE Geobacillus stearothermophilus
3.2.1.55 256000
-
-
 Geobacillus stearothermophilus
3.2.1.55 284000
-
 gel filtration Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.55 beet arabinan + H2O Geobacillus stearothermophilus
-
 L-arabinose + ?
-
 ?
3.2.1.55 beet arabinan + H2O Aspergillus niger
-
 L-arabinose + ?
-
 ?
3.2.1.55 beet arabinan + H2O Ruminococcus albus
-
 L-arabinose + ?
-
 ?
3.2.1.55 beet arabinan + H2O Butyrivibrio fibrisolvens
-
 L-arabinose + ?
-
 ?
3.2.1.55 beet arabinan + H2O Clostridium acetobutylicum
-
 L-arabinose + ?
-
 ?
3.2.1.55 beet arabinan + H2O Streptomyces lividans
-
 L-arabinose + ?
-
 ?
3.2.1.55 beet arabinan + H2O Streptomyces purpurascens
-
 L-arabinose + ?
-
 ?
3.2.1.55 beet arabinan + H2O Ruminococcus albus 8
-
 L-arabinose + ?
-
 ?
3.2.1.55 beet arabinan + H2O Butyrivibrio fibrisolvens GS113
-
 L-arabinose + ?
-
 ?
3.2.1.55 beet arabinan + H2O Streptomyces lividans 66
-
 L-arabinose + ?
-
 ?
3.2.1.55 beet arabinan + H2O Streptomyces lividans 66 1326
-
 L-arabinose + ?
-
 ?
3.2.1.55 beet arabinan + H2O Geobacillus stearothermophilus L1
-
 L-arabinose + ?
-
 ?
3.2.1.55 beet arabinan + H2O Geobacillus stearothermophilus T-6
-
 L-arabinose + ?
-
 ?
3.2.1.55 oat spelt xylan + H2O Geobacillus stearothermophilus
-
 L-arabinose + ?
-
 ?
3.2.1.55 oat spelt xylan + H2O Aspergillus niger
-
 L-arabinose + ?
-
 ?
3.2.1.55 oat spelt xylan + H2O Butyrivibrio fibrisolvens
-
 L-arabinose + ?
-
 ?
3.2.1.55 oat spelt xylan + H2O Clostridium acetobutylicum
-
 L-arabinose + ?
-
 ?
3.2.1.55 oat spelt xylan + H2O Trichoderma reesei
-
 L-arabinose + ?
-
 ?
3.2.1.55 oat spelt xylan + H2O Streptomyces diastaticus
-
 L-arabinose + ?
-
 ?
3.2.1.55 oat spelt xylan + H2O Butyrivibrio fibrisolvens GS113
-
 L-arabinose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.55 Aspergillus niger
-
-
-
3.2.1.55 Butyrivibrio fibrisolvens
-
 GS113
-
3.2.1.55 Butyrivibrio fibrisolvens GS113
-
 GS113
-
3.2.1.55 Clostridium acetobutylicum
-
 ATCC 824
-
3.2.1.55 Geobacillus stearothermophilus
-
 L1
-
3.2.1.55 Geobacillus stearothermophilus
-
 T-6
-
3.2.1.55 Geobacillus stearothermophilus L1
-
 L1
-
3.2.1.55 Geobacillus stearothermophilus T-6
-
 T-6
-
3.2.1.55 Ruminococcus albus
-
 8
-
3.2.1.55 Ruminococcus albus 8
-
 8
-
3.2.1.55 Sclerotinia sclerotiorum
-
-
-
3.2.1.55 Streptomyces diastaticus
-
-
-
3.2.1.55 Streptomyces lividans
-
 66
-
3.2.1.55 Streptomyces lividans
-
 66strain 1326
-
3.2.1.55 Streptomyces lividans 66
-
 66
-
3.2.1.55 Streptomyces lividans 66 1326
-
 66strain 1326
-
3.2.1.55 Streptomyces purpurascens
-
 IFO 3389
-
3.2.1.55 Trichoderma reesei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.55
-
 Geobacillus stearothermophilus
3.2.1.55
-
 Ruminococcus albus
3.2.1.55
-
 Butyrivibrio fibrisolvens
3.2.1.55
-
 Streptomyces lividans
3.2.1.55
-
 Streptomyces diastaticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.55 95
-
 extracellular enzyme activity Geobacillus stearothermophilus
3.2.1.55 95
-
 extracellular enzyme activity Butyrivibrio fibrisolvens
3.2.1.55 749.1
-
-
 Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.55 beet arabinan + H2O
-
 Geobacillus stearothermophilus L-arabinose + ?
-
 ?
3.2.1.55 beet arabinan + H2O
-
 Aspergillus niger L-arabinose + ?
-
 ?
3.2.1.55 beet arabinan + H2O
-
 Ruminococcus albus L-arabinose + ?
-
 ?
3.2.1.55 beet arabinan + H2O
-
 Butyrivibrio fibrisolvens L-arabinose + ?
-
 ?
3.2.1.55 beet arabinan + H2O
-
 Clostridium acetobutylicum L-arabinose + ?
-
 ?
3.2.1.55 beet arabinan + H2O
-
 Streptomyces lividans L-arabinose + ?
-
 ?
3.2.1.55 beet arabinan + H2O
-
 Streptomyces purpurascens L-arabinose + ?
-
 ?
3.2.1.55 beet arabinan + H2O
-
 Ruminococcus albus 8 L-arabinose + ?
-
 ?
3.2.1.55 beet arabinan + H2O
-
 Butyrivibrio fibrisolvens GS113 L-arabinose + ?
-
 ?
3.2.1.55 beet arabinan + H2O
-
 Streptomyces lividans 66 L-arabinose + ?
-
 ?
3.2.1.55 beet arabinan + H2O
-
 Streptomyces lividans 66 1326 L-arabinose + ?
-
 ?
3.2.1.55 beet arabinan + H2O
-
 Geobacillus stearothermophilus L1 L-arabinose + ?
-
 ?
3.2.1.55 beet arabinan + H2O
-
 Geobacillus stearothermophilus T-6 L-arabinose + ?
-
 ?
3.2.1.55 carboxymethyl cellulose + H2O
-
 Geobacillus stearothermophilus ?
-
 ?
3.2.1.55 carboxymethyl cellulose + H2O
-
 Geobacillus stearothermophilus L1 ?
-
 ?
3.2.1.55 carboxymethyl cellulose + H2O
-
 Geobacillus stearothermophilus T-6 ?
-
 ?
3.2.1.55 L-arabinan + H2O
-
 Geobacillus stearothermophilus (1,5-alpha-L-arabinose)25 + ?
-
 ?
3.2.1.55 L-arabinan + H2O
-
 Aspergillus niger (1,5-alpha-L-arabinose)25 + ?
-
 ?
3.2.1.55 L-arabinan + H2O
-
 Clostridium acetobutylicum (1,5-alpha-L-arabinose)25 + ?
-
 ?
3.2.1.55 L-arabinan + H2O
-
 Streptomyces lividans (1,5-alpha-L-arabinose)25 + ?
-
 ?
3.2.1.55 L-arabinan + H2O
-
 Streptomyces purpurascens (1,5-alpha-L-arabinose)25 + ?
-
 ?
3.2.1.55 L-arabinan + H2O
-
 Streptomyces lividans 66 (1,5-alpha-L-arabinose)25 + ?
-
 ?
3.2.1.55 L-arabinan + H2O
-
 Streptomyces lividans 66 1326 (1,5-alpha-L-arabinose)25 + ?
-
 ?
3.2.1.55 L-arabinan + H2O
-
 Geobacillus stearothermophilus L1 (1,5-alpha-L-arabinose)25 + ?
-
 ?
3.2.1.55 L-arabinan + H2O
-
 Geobacillus stearothermophilus T-6 (1,5-alpha-L-arabinose)25 + ?
-
 ?
3.2.1.55 additional information
-
 Aspergillus niger ?
-
 ?
3.2.1.55 additional information
-
 Butyrivibrio fibrisolvens ?
-
 ?
3.2.1.55 additional information
-
 Clostridium acetobutylicum ?
-
 ?
3.2.1.55 additional information
-
 Streptomyces lividans ?
-
 ?
3.2.1.55 additional information
-
 Streptomyces purpurascens ?
-
 ?
3.2.1.55 additional information no activity detected with beta-L-arabinopyranoside, beta-D-cellobiose, alpha-L-fucopyranoside, beta-L-fucopyranoside, alpha-L-rhamnopyranoside, alpha-L-arabinopyranoside, beta-D-galactopyranoside, beta-D-glucopyranoside, alpha-D-mannopyranoside, beta-D-xylopyranoside and arabinogalactan Geobacillus stearothermophilus ?
-
 ?
3.2.1.55 additional information
-
 Butyrivibrio fibrisolvens GS113 ?
-
 ?
3.2.1.55 additional information
-
 Streptomyces lividans 66 ?
-
 ?
3.2.1.55 additional information
-
 Streptomyces lividans 66 1326 ?
-
 ?
3.2.1.55 additional information no activity detected with beta-L-arabinopyranoside, beta-D-cellobiose, alpha-L-fucopyranoside, beta-L-fucopyranoside, alpha-L-rhamnopyranoside, alpha-L-arabinopyranoside, beta-D-galactopyranoside, beta-D-glucopyranoside, alpha-D-mannopyranoside, beta-D-xylopyranoside and arabinogalactan Geobacillus stearothermophilus L1 ?
-
 ?
3.2.1.55 additional information no activity detected with beta-L-arabinopyranoside, beta-D-cellobiose, alpha-L-fucopyranoside, beta-L-fucopyranoside, alpha-L-rhamnopyranoside, alpha-L-arabinopyranoside, beta-D-galactopyranoside, beta-D-glucopyranoside, alpha-D-mannopyranoside, beta-D-xylopyranoside and arabinogalactan Geobacillus stearothermophilus T-6 ?
-
 ?
3.2.1.55 oat spelt xylan + H2O
-
 Geobacillus stearothermophilus L-arabinose + ?
-
 ?
3.2.1.55 oat spelt xylan + H2O
-
 Aspergillus niger L-arabinose + ?
-
 ?
3.2.1.55 oat spelt xylan + H2O
-
 Butyrivibrio fibrisolvens L-arabinose + ?
-
 ?
3.2.1.55 oat spelt xylan + H2O
-
 Clostridium acetobutylicum L-arabinose + ?
-
 ?
3.2.1.55 oat spelt xylan + H2O
-
 Trichoderma reesei L-arabinose + ?
-
 ?
3.2.1.55 oat spelt xylan + H2O
-
 Streptomyces diastaticus L-arabinose + ?
-
 ?
3.2.1.55 oat spelt xylan + H2O
-
 Butyrivibrio fibrisolvens GS113 L-arabinose + ?
-
 ?
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Geobacillus stearothermophilus L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Aspergillus niger L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Ruminococcus albus L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Butyrivibrio fibrisolvens L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Sclerotinia sclerotiorum L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Clostridium acetobutylicum L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Trichoderma reesei L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Streptomyces lividans L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Streptomyces purpurascens L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Streptomyces diastaticus L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Ruminococcus albus 8 L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Butyrivibrio fibrisolvens GS113 L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Streptomyces lividans 66 L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Streptomyces lividans 66 1326 L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Geobacillus stearothermophilus L1 L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Geobacillus stearothermophilus T-6 L-arabinose + phenol
-
 ir

Subunits

EC Number Subunits Comment Organism
3.2.1.55 dimer strain L1 Geobacillus stearothermophilus
3.2.1.55 octamer
-
 Butyrivibrio fibrisolvens
3.2.1.55 octamer
-
 Streptomyces purpurascens
3.2.1.55 tetramer
-
 Ruminococcus albus
3.2.1.55 tetramer 4 * 64000, SDS-PAGE Geobacillus stearothermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.55 70
-
-
 Geobacillus stearothermophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.55 45 80
-
 Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.55 5.5 6
-
 Geobacillus stearothermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.55 4 10
-
 Geobacillus stearothermophilus