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Literature summary extracted from

  • Lee, S.F.; Forsberg, C.W.
    Purification and characterization of an alpha-L-arabinofuranosidase from Clostridium acetobutylicum ATCC 824 (1987), Can. J. Microbiol., 33, 1011-1016.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.55 0.082
-
 p-nitrophenyl-alpha-L-arabinofuranoside
-
 Streptomyces purpurascens
3.2.1.55 0.26
-
 beet arabinan
-
 Aspergillus niger
3.2.1.55 1.6
-
 p-nitrophenyl-alpha-L-arabinofuranoside
-
 Ruminococcus albus
3.2.1.55 2
-
 beet arabinan
-
 Clostridium acetobutylicum
3.2.1.55 3.6
-
 p-nitrophenyl-alpha-L-arabinofuranoside
-
 Streptomyces sp.
3.2.1.55 4
-
 p-nitrophenyl-alpha-L-arabinofuranoside
-
 Ruminococcus albus
3.2.1.55 4
-
 p-nitrophenyl-alpha-L-arabinofuranoside
-
 Clostridium acetobutylicum
3.2.1.55 4.9
-
 p-nitrophenyl-alpha-L-arabinofuranoside
-
 Aspergillus niger

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.55 extracellular
-
 Aspergillus niger
-
-
3.2.1.55 extracellular
-
 Ruminococcus albus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.55 53000
-
-
 Aspergillus niger
3.2.1.55 62000
-
 8 * 62000 Streptomyces purpurascens
3.2.1.55 65000
-
-
 Bacillus subtilis
3.2.1.55 75000
-
 4 * 75000, SDS-PAGE Ruminococcus albus
3.2.1.55 90000
-
 SDS-PAGE Clostridium acetobutylicum
3.2.1.55 92000
-
-
 Streptomyces sp.
3.2.1.55 94000
-
 gel filtration Clostridium acetobutylicum
3.2.1.55 310000
-
-
 Ruminococcus albus
3.2.1.55 495000
-
-
 Streptomyces purpurascens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.55 beet arabinan + H2O Bacillus subtilis
-
 L-arabinose + ?
-
 ir
3.2.1.55 beet arabinan + H2O Aspergillus niger
-
 L-arabinose + ?
-
 ir
3.2.1.55 beet arabinan + H2O Ruminococcus albus
-
 L-arabinose + ?
-
 ir
3.2.1.55 beet arabinan + H2O Streptomyces sp.
-
 L-arabinose + ?
-
 ir
3.2.1.55 beet arabinan + H2O Clostridium acetobutylicum
-
 L-arabinose + ?
-
 ir
3.2.1.55 beet arabinan + H2O Streptomyces purpurascens
-
 L-arabinose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.55 Aspergillus niger
-
-
-
3.2.1.55 Bacillus subtilis
-
-
-
3.2.1.55 Clostridium acetobutylicum
-
 ATCC 824
-
3.2.1.55 Ruminococcus albus
-
-
-
3.2.1.55 Streptomyces purpurascens
-
-
-
3.2.1.55 Streptomyces sp.
-
 no. 17-1
-
3.2.1.55 Streptomyces sp. 17-1
-
 no. 17-1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.55
-
 Bacillus subtilis
3.2.1.55
-
 Ruminococcus albus
3.2.1.55
-
 Clostridium acetobutylicum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.55 1.8
-
 p-nitrophenyl-alpha-D-glucoside as substrate Clostridium acetobutylicum
3.2.1.55 19.2
-
-
 Clostridium acetobutylicum
3.2.1.55 19.4
-
 p-nitrophenyl-alpha-L-arabinofuranoside as substrate Clostridium acetobutylicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.55 1,5-alpha-L-arabinobiose + H2O
-
 Streptomyces purpurascens L-arabinose
-
 ?
3.2.1.55 1,5-alpha-L-arabinotriose + 2 H2O
-
 Aspergillus niger 3 L-arabinose
-
 ?
3.2.1.55 1,5-alpha-L-arabinotriose + 2 H2O
-
 Streptomyces purpurascens 3 L-arabinose
-
 ?
3.2.1.55 4-nitrophenyl alpha-D-glucoside + H2O 9% of the rate exhibited on p-nitrophenyl-alpha-L-arabinofuranoside Clostridium acetobutylicum 4-nitrophenol + D-glucose
-
 ?
3.2.1.55 base-soluble carbohydrates + H2O BSC of alfalfa cell walls Ruminococcus albus ?
-
 ?
3.2.1.55 beet arabinan + H2O
-
 Bacillus subtilis L-arabinose + ?
-
 ir
3.2.1.55 beet arabinan + H2O
-
 Aspergillus niger L-arabinose + ?
-
 ir
3.2.1.55 beet arabinan + H2O
-
 Ruminococcus albus L-arabinose + ?
-
 ir
3.2.1.55 beet arabinan + H2O
-
 Streptomyces sp. L-arabinose + ?
-
 ir
3.2.1.55 beet arabinan + H2O
-
 Clostridium acetobutylicum L-arabinose + ?
-
 ir
3.2.1.55 beet arabinan + H2O
-
 Streptomyces purpurascens L-arabinose + ?
-
 ?
3.2.1.55 L-arabinan + H2O
-
 Bacillus subtilis (1,5-alpha-L-arabinose)25 + ?
-
 ir
3.2.1.55 L-arabinan + H2O
-
 Aspergillus niger (1,5-alpha-L-arabinose)25 + ?
-
 ir
3.2.1.55 L-arabinan + H2O
-
 Clostridium acetobutylicum (1,5-alpha-L-arabinose)25 + ?
-
 ir
3.2.1.55 L-arabinan + H2O
-
 Streptomyces purpurascens (1,5-alpha-L-arabinose)25 + ?
-
 ?
3.2.1.55 L-arabinogalactan + H2O
-
 Aspergillus niger L-arabinose + ?
-
 ?
3.2.1.55 L-arabinogalactan + H2O
-
 Streptomyces sp. L-arabinose + ?
-
 ?
3.2.1.55 L-arabinogalactan + H2O
-
 Streptomyces sp. 17-1 L-arabinose + ?
-
 ?
3.2.1.55 L-arabinoxylan + H2O
-
 Aspergillus niger L-arabinose + ?
-
 ir
3.2.1.55 L-arabinoxylan + H2O
-
 Streptomyces sp. L-arabinose + ?
-
 ir
3.2.1.55 L-arabinoxylan + H2O
-
 Streptomyces sp. 17-1 L-arabinose + ?
-
 ir
3.2.1.55 additional information
-
 Bacillus subtilis ?
-
 ?
3.2.1.55 additional information
-
 Aspergillus niger ?
-
 ?
3.2.1.55 additional information
-
 Streptomyces sp. ?
-
 ?
3.2.1.55 additional information
-
 Streptomyces purpurascens ?
-
 ?
3.2.1.55 additional information no activity against p-nitrophenylglycosides, except p-nitrophenyl-alpha-D-glucoside, no activity on carboxymethylcellulose, arabinogalactan, arabinoxylan or oat spelt xylan, larchwood xylan, CMC and rye arabinoxylan are not degraded Clostridium acetobutylicum ?
-
 ?
3.2.1.55 additional information
-
 Streptomyces sp. 17-1 ?
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Bacillus subtilis p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Aspergillus niger p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Ruminococcus albus p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Streptomyces sp. p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Clostridium acetobutylicum p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Streptomyces purpurascens p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 p-nitrophenyl-alpha-L-arabinofuranoside + H2O
-
 Streptomyces sp. 17-1 p-nitrophenol + L-arabinose
-
 ?
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Bacillus subtilis L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Aspergillus niger L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Ruminococcus albus L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Streptomyces sp. L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Clostridium acetobutylicum L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Streptomyces purpurascens L-arabinose + phenol
-
 ir
3.2.1.55 phenyl alpha-L-arabinofuranoside + H2O
-
 Streptomyces sp. 17-1 L-arabinose + phenol
-
 ir
3.2.1.55 polygalacturonase-soluble carbohydrates + H2O PGSC of alfalfa cell walls Ruminococcus albus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.55 monomer
-
 Bacillus subtilis
3.2.1.55 monomer
-
 Aspergillus niger
3.2.1.55 monomer
-
 Streptomyces sp.
3.2.1.55 monomer 1 * 90000, SDS-PAGE Clostridium acetobutylicum
3.2.1.55 octamer 8 * 62000 Streptomyces purpurascens
3.2.1.55 tetramer 4 * 75000, SDS-PAGE Ruminococcus albus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.55 3.8 4
-
 Aspergillus niger
3.2.1.55 5 5.5
-
 Clostridium acetobutylicum
3.2.1.55 6
-
-
 Streptomyces sp.
3.2.1.55 6.5
-
-
 Bacillus subtilis
3.2.1.55 6.5
-
-
 Streptomyces purpurascens
3.2.1.55 6.9
-
-
 Ruminococcus albus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.55 3 4 poor stability at low pH Clostridium acetobutylicum
3.2.1.55 4 9
-
 Streptomyces sp.
3.2.1.55 5.5 8
-
 Clostridium acetobutylicum