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Literature summary extracted from

  • Böttcher, F.; Ober, D.; Hartmann, T.
    Biosynthesis of pyrrolizidine alkaloids: putrescine and spermidine are essential substrates of enzymatic homospermidine formation (1994), Can. J. Chem., 72, 80-85.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.44 0.26
-
 putrescine
-
 Blastochloris viridis
2.5.1.44 1.5
-
 spermidine in presence of 1 mM putrescine Blastochloris viridis
2.5.1.44 1.7
-
 putrescine in presence of 1 mM spermidine Blastochloris viridis
2.5.1.45 0.012
-
 spermidine at 0.1 mM putrescine Eupatorium cannabinum
2.5.1.45 0.015
-
 putrescine at 0.1 mM spermidine Eupatorium cannabinum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.44 2 putrescine Blastochloris viridis
-
 sym-homospermidine + NH3
-
 ?
2.5.1.45 putrescine + spermine Eupatorium cannabinum
-
 sym-homospermine + propane-1,3-diamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.44 Blastochloris viridis
-
 DSM 134
-
2.5.1.44 Blastochloris viridis
-
 accession no. L77975
-
2.5.1.45 Eupatorium cannabinum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.44 partial Blastochloris viridis
2.5.1.45
-
 Eupatorium cannabinum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.44 culture medium
-
 Blastochloris viridis
-
2.5.1.45 root
-
 Eupatorium cannabinum
-
2.5.1.45 tissue culture
-
 Eupatorium cannabinum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.44 0.6 1.2
-
 Blastochloris viridis
2.5.1.45 0.0006
-
-
 Eupatorium cannabinum

Storage Stability

EC Number Storage Stability Organism
2.5.1.44 -18°C, storage as acetone dry-powder, without significant loss of activity Blastochloris viridis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.44 2 putrescine
-
 Blastochloris viridis sym-homospermidine + NH3
-
 ?
2.5.1.44 putrescine + 1,3-diaminopropane
-
 Blastochloris viridis homospermidine + spermidine low but significant amounts of homologous polyamines in addition to homospermidine ?
2.5.1.44 putrescine + 1,6 diaminohexane
-
 Blastochloris viridis homospermidine + N-(4-aminobutyl)-1,6-diaminohexane low but significant amounts of homologous polyamines in addition to homospermidine ?
2.5.1.44 putrescine + 1,7-diaminoheptane
-
 Blastochloris viridis homospermidine + N-(4-aminobutyl)-1,7-diaminoheptane low but significant amounts of homologous polyamines in addition to homospermidine ?
2.5.1.44 putrescine + cadaverine
-
 Blastochloris viridis homospermidine + N-(4-aminobutyl)-1,5-diaminopentane low but significant amounts of homologous polyamines in addition to homospermidine ?
2.5.1.44 spermidine
-
 Blastochloris viridis homospermidine + putrescine + 1,3-diaminopropane
-
 ?
2.5.1.44 spermidine + 1,6-diaminohexane
-
 Blastochloris viridis homospermidine + N-(4-aminobutyl)-1,6-diaminohexane
-
 ?
2.5.1.44 spermidine + cadaverine
-
 Blastochloris viridis homospermidine + N-(4-aminobutyl)-1,5-diaminopentane
-
 ?
2.5.1.44 spermidine + putrescine
-
 Blastochloris viridis homospermidine + 1,3-diaminopropane
-
 ?
2.5.1.45 putrescine + spermidine
-
 Eupatorium cannabinum N-(4-aminobutyl)butane-1,4-diamine + propane-1,3-diamine
-
 ?
2.5.1.45 putrescine + spermine
-
 Eupatorium cannabinum sym-homospermine + propane-1,3-diamine
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.44 4
-
 stable for at least several weeks Blastochloris viridis

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.44 NAD+ NAD+ seems to function as hydride acceptor in the first part of the reaction and subsequently as hydride donor in the second part Blastochloris viridis
2.5.1.45 NAD+
-
 Eupatorium cannabinum
2.5.1.45 NADH
-
 Eupatorium cannabinum