EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.6.2.4 | additional information | storage of the enzyme at -75°C for 4 months results in a slight activation of the enzyme activity | Petunia x hybrida | |
1.6.2.4 | potassium phosphate | activity slightly higher than with sodium chloride or sodium sulfate | Petunia x hybrida |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.6.2.4 | endoplasmic reticulum | - |
Petunia x hybrida | 5783 | - |
1.6.2.4 | microsome | - |
Petunia x hybrida | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.6.2.4 | 84000 | - |
Western blot analysis of fresh microsomal extracts | Petunia x hybrida |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.6.2.4 | Petunia x hybrida | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.6.2.4 | using column chromatography on adenosine 2',5'-biphosphate-Sepharose, followed by high-performance anion-exchange chromatography | Petunia x hybrida |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.6.2.4 | flower | buds and nascent, open flowers | Petunia x hybrida | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.6.2.4 | 0.02 | 0.12 | flower buds, open flowers exhibits a similar value | Petunia x hybrida |
1.6.2.4 | 60 | 120 | purified enzyme | Petunia x hybrida |
EC Number | Storage Stability | Organism |
---|---|---|
1.6.2.4 | -75°C, months, 5 mM 2'-AMP | Petunia x hybrida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.6.2.4 | 2 ferricytochrome c + NADPH | additional electron acceptor: neotetrazolium chloride | Petunia x hybrida | 2 ferrocytochrome c + NADP+ + H+ | - |
? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.6.2.4 | 7.4 | - |
- |
Petunia x hybrida |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.6.2.4 | 6 | 8 | - |
Petunia x hybrida |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.6.2.4 | FAD | - |
Petunia x hybrida | |
1.6.2.4 | FMN | - |
Petunia x hybrida | |
1.6.2.4 | NADH | activity not detected | Petunia x hybrida | |
1.6.2.4 | NADPH | - |
Petunia x hybrida |