EC Number | Cloned (Comment) | Organism |
---|---|---|
1.2.1.65 | expression in Escherichia coli and Pseudomonas aeruginosa | Pseudomonas putida |
4.1.2.45 | expression in Escherichia coli | Pseudomonas putida |
5.99.1.4 | expression in Escherichia coli | Pseudomonas putida |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.1.2.45 | additional information | not sensitive to 5 mM EDTA | Pseudomonas putida |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.65 | salicylaldehyde + NAD+ + H2O | Pseudomonas putida | - |
salicylate + NADH | - |
? | |
1.2.1.65 | salicylaldehyde + NAD+ + H2O | Pseudomonas putida PpG1064 | - |
salicylate + NADH | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.65 | Pseudomonas putida | - |
- |
- |
1.2.1.65 | Pseudomonas putida PpG1064 | - |
- |
- |
4.1.2.45 | Pseudomonas putida | Q51947 | - |
- |
5.99.1.4 | Pseudomonas putida | Q51948 | - |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.2.1.65 | cell culture | - |
Pseudomonas putida | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.1.2.45 | 1.55 | - |
recombinant enzyme | Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.65 | salicylaldehyde + NAD+ + H2O | - |
Pseudomonas putida | salicylate + NADH | - |
? | |
1.2.1.65 | salicylaldehyde + NAD+ + H2O | - |
Pseudomonas putida PpG1064 | salicylate + NADH | - |
? | |
4.1.2.45 | (E)-2'-hydroxybenzylidenepyruvate + O2 | the equilibrium in this reaction favors cleavage | Pseudomonas putida | salicylaldehyde + pyruvate | - |
r | |
4.1.2.45 | additional information | no activity with trans-benzylidenepyruvate, trans-o-methoxybenzylidenepyruvate or trans-o-hydroxycinnamate. The hydratase-aldolase catalyzes the condensation of pyruvate with several other aromatic aldehydes, including benzaldehyde, to give trans-benzylidenepyruvate. Since benzylidenepyruvate is not a substrate for the enzyme, the reaction is irreversible and can be carried out to completion by using relatively low concentrations of the substrates, benzaldehyde and pyruvate | Pseudomonas putida | ? | - |
? | |
5.99.1.4 | 2-hydroxy-2H-chromene-2-carboxylate | extracts of Escherichia coli JM109 carrying pRE718 catalyze the conversion of trans-o-hydroxybenzylidenepyruvate or 2-hydroxychromene-2-carboxylate to an equilibrium mixture that contained 55% 2-hydroxychromene-2-carboxylate and 45% trans-o-hydroxybenzylidenepyruvate at pH 7. At pH 10 the reaction occus entirely in on direction, the conversion of 2-hydroxychromene-2-carboxylate to trans-o-hydroxybenzylidenepyruvate. The product is identified by nuclear magnetic resonance spectroscopy. This isomerization occurs spontaneously, although at a slower rate than the enzyme-catalyzed reaction | Pseudomonas putida | (3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate | i.e. (E)-2'-hydroxybenzylidenepyruvate, i.e. trans-o-hydroxybenzylidenepyruvate | r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.2.45 | tHBPA hydratase-aldolase | - |
Pseudomonas putida |
5.99.1.4 | 2-hydroxychromene-2-carboxylate isomerase | - |
Pseudomonas putida |
5.99.1.4 | HCCA isomerase | - |
Pseudomonas putida |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.65 | NAD+ | - |
Pseudomonas putida | |
4.1.2.45 | additional information | no cofactor required | Pseudomonas putida |