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Literature summary extracted from

  • Maruyama, K.; Ariga, N.; Tsuda, M.; Deguchi, K.
    Purification and properties of alpha-hydroxy-gamma-carboxymuconic epsilon-semialdehyde dehydrogenase (1978), J. Biochem., 83, 1125-1134.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.312 HgCl2
-
 Pseudomonas straminea
1.1.1.312 Monoiodoacetic acid weaker inhibitor than p-chloromercuribenzoate or HgCl2 Pseudomonas straminea
1.1.1.312 additional information not: various metal ions, metal chelating reagents, reducing reagents Pseudomonas straminea
1.1.1.312 N-ethylmaleimide weaker inhibitor than p-chloromercuribenzoate or HgCl2 Pseudomonas straminea
1.1.1.312 p-chloromercuribenzoate
-
 Pseudomonas straminea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.312 0.0175
-
 NADP+
-
 Pseudomonas straminea
1.1.1.312 0.02
-
 4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde in the presence of 0.110 mM NADP+ Pseudomonas straminea
1.1.1.312 0.056
-
 4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde in the presence of 0.110 mM NAD+ Pseudomonas straminea
1.1.1.312 0.284
-
 NAD+
-
 Pseudomonas straminea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.312 35000
-
 2 * 35000, SDS-PAGE Pseudomonas straminea
1.1.1.312 67000
-
 gel filtration Pseudomonas straminea

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.312 Pseudomonas straminea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.312 using gel filtration on Sephadex G-200 Pseudomonas straminea

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.312 0.413
-
-
 Pseudomonas straminea

Storage Stability

EC Number Storage Stability Organism
1.1.1.312 -20°C, for at least 4 months Pseudomonas straminea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.312 4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde + NAD+
-
 Pseudomonas straminea 4-carboxy-2-hydroxy-cis,cis-muconate + NADH
-
 ?
1.1.1.312 4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde + NADP+ or NAD+ Pseudomonas straminea 4-carboxy-2-hydroxy-cis,cis-muconate + NADPH
-
 ?
1.1.1.312 additional information not: unsubstituted aliphatic or aromatic aldehydes Pseudomonas straminea ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.312 dimer 2 * 35000, SDS-PAGE Pseudomonas straminea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.312 24
-
 assay at Pseudomonas straminea

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.312 42
-
 10 min, pH 7.0, 50% loss of activity Pseudomonas straminea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.312 7 8
-
 Pseudomonas straminea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.312 5.5 9.3 at pH 5.5 and 9.3: about 50% of activity maximum Pseudomonas straminea

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.312 6.5 7 highest stability Pseudomonas straminea

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.312 NAD+ can replace NADP+, but with lower affinity Pseudomonas straminea
1.1.1.312 NADP+
-
 Pseudomonas straminea