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Literature summary extracted from

  • Ray, S.; Ray, M.
    Purification and characterization of NAD and NADP-linked alpha-ketoaldehyde dehydrogenases involved in catalyzing the oxidation of methylglyoxal to pyruvate (1982), J. Biol. Chem., 257, 10566-10570.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.23 beta-mercaptoethanol restores the activity after inactivation with p-chloromercuribenzoate Capra hircus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.23 5,5'-dithiobis(2-nitrobenzoate)
-
 Capra hircus
1.2.1.23 N-ethylmaleimide 1 mM Capra hircus
1.2.1.23 p-chloromercuribenzoate
-
 Capra hircus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.23 0.19
-
 NAD+
-
 Capra hircus
1.2.1.23 1.7
-
 methylglyoxal
-
 Capra hircus
1.2.1.49 0.04
-
 NADP+
-
 Capra hircus
1.2.1.49 0.21 0.83 methylglyoxal
-
 Capra hircus
1.2.1.49 0.5
-
 methylglyoxal
-
 Capra hircus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.23 42000
-
 gel filtration Capra hircus
1.2.1.23 43000
-
 1 * 43000, SDS-PAGE Capra hircus
1.2.1.49 42000
-
 gel filtration Capra hircus
1.2.1.49 43000
-
 1 * 43000, SDS-PAGE Capra hircus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.23 methylglyoxal + NAD+ + H2O Capra hircus i.e. pyruvaldehyde pyruvate + NADH + H+
-
 ?
1.2.1.49 methylglyoxal + NADP+ + H2O Capra hircus
-
 pyruvate + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.23 Capra hircus
-
-
-
1.2.1.49 Capra hircus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.23 using acetone powder extract, ammonium sulfate precipitation, calcium phosphate gel treatment, column chromatography on DEAE-cellulose and NAD-Agarose, separated from the NADP-dependent enzyme through affinity chromatography on a thiol-Sepharose column Capra hircus
1.2.1.49
-
 Capra hircus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.23 liver
-
 Capra hircus
-
1.2.1.49 liver
-
 Capra hircus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.23 18.4
-
-
 Capra hircus
1.2.1.49 2.6
-
-
 Capra hircus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.23 2-oxoaldehydes + NAD+ + H2O
-
 Capra hircus 2-oxo acids + NADH
-
 ?
1.2.1.23 aldehydes + NAD+ + H2O
-
 Capra hircus carboxylic acids + NADH
-
 ?
1.2.1.23 methylglyoxal + NAD+ + H2O i.e. pyruvaldehyde Capra hircus pyruvate + NADH + H+
-
 ?
1.2.1.49 2-oxoaldehydes + NADP+ + H2O
-
 Capra hircus 2-oxo acids + NADPH
-
 ?
1.2.1.49 methylglyoxal + NADP+ + H2O
-
 Capra hircus pyruvate + NADPH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.23 monomer 1 * 43000, SDS-PAGE Capra hircus
1.2.1.49 monomer 1 * 43000, SDS-PAGE Capra hircus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.23 8
-
-
 Capra hircus
1.2.1.49 8
-
-
 Capra hircus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.23 NAD+
-
 Capra hircus
1.2.1.49 NADP+
-
 Capra hircus