Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Galkin, A.G.; Kutsenko, A.S.; Bajulina, N.P.; Esipova, N.G.; Lamzin, V.S.; Mesentsev, A.V.; Shelukho, D.V.; Tikhonova, T.V.; Tishkov, V.I.; Ustinnikova, T.B.; Popov, V.O.
    Site-directed mutagenesis of the essential arginine of the formate dehydrogenase active centre (2001), Biochim. Biophys. Acta, 1594, 136-149.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.17.1.9 R284A change of the catalytic, thermodynamic and spectral properties of the enzyme. The affinity of the mutants for the substrate formate or the transition state analogue azide decreases Pseudomonas sp.
1.17.1.9 R284A mutant enzyme is completely inactive Pseudomonas sp.
1.17.1.9 R284Q change of the catalytic, thermodynamic and spectral properties of the enzyme. The affinity of the mutants for the substrate formate or the transition state analogue azide decreases Pseudomonas sp.
1.17.1.9 R284Q Km-value for formate and Ki-value for azide decreases, KM-value for NAD+ is not affected Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.1.9 azide
-
 Pseudomonas sp.
1.17.1.9 N3-
-
 Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.1.9 0.11
-
 NAD+ pH 7.0, 37°C, wild-type enzyme Pseudomonas sp.
1.17.1.9 0.17
-
 formate pH 7.0, 37°C, mutant enzyme R284Q Pseudomonas sp.
1.17.1.9 15
-
 formate pH 7.0, 37°C, wild-type enzyme Pseudomonas sp.
1.17.1.9 145
-
 formate pH 7.0, 37°C, mutant enzyme R284Q Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.9 Pseudomonas sp.
-
-
-

Synonyms

EC Number Synonyms Comment Organism
1.17.1.9 formate dehydrogenase
-
 Pseudomonas sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.17.1.9 0.31
-
 formate pH 7.0, 37°C, mutant enzyme R284Q Pseudomonas sp.
1.17.1.9 10
-
 formate pH 7.0, 37°C, wild-type enzyme Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.9 NAD+
-
 Pseudomonas sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.17.1.9 0.00015
-
 azide pH 7.0, 37°C, wild-type enzyme Pseudomonas sp.
1.17.1.9 0.024
-
 azide pH 7.0, 37°C, mutant enzyme R284Q Pseudomonas sp.