Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hensel, R.; Laumann, S.; Lang, J.; Heumann, H.; Lottspeich, F.
    Characterization of two D-glyceraldehyde-3-phosphate dehydrogenases from the extremely thermophilic archaebacterium Thermoproteus tenax (1987), Eur. J. Biochem., 170, 325-333.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.2.1.13 the enzyme is stabilized by glycerol, several salts, especially sodium or potassium phosphate Thermoproteus tenax

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.13 0.027
-
 NADP+
-
 Thermoproteus tenax
1.2.1.13 30
-
 arsenate
-
 Thermoproteus tenax
1.2.1.13 50
-
 phosphate
-
 Thermoproteus tenax

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.13 39000
-
 x * 39000, SDS-PAGE Thermoproteus tenax
1.2.1.13 39000
-
 4 * 39000, SDS-PAGE Thermoproteus tenax
1.2.1.13 210000
-
 gel filtration Thermoproteus tenax

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.13 Thermoproteus tenax
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.13 17
-
-
 Thermoproteus tenax

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.13 D-glyceraldehyde 3-phosphate + arsenate + NADP+
-
 Thermoproteus tenax 3-phospho-D-glyceroyl arsenate + NADPH
-
 ?
1.2.1.13 D-glyceraldehyde 3-phosphate + phosphate + NADP+
-
 Thermoproteus tenax 3-phospho-D-glyceroyl phosphate + NADPH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.13 ? x * 39000, SDS-PAGE Thermoproteus tenax
1.2.1.13 tetramer 4 * 39000, SDS-PAGE Thermoproteus tenax

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.13 96
-
 half-life: 130 min Thermoproteus tenax
1.2.1.13 100
-
 half-life: 35 min Thermoproteus tenax

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.13 7
-
 oxidation of D-glyceraldehyde-3-phosphate Thermoproteus tenax
1.2.1.13 8
-
 reduction of 3-phospho-D-glyceroyl phosphate Thermoproteus tenax

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.13 NADP+ cofactor Thermoproteus tenax